Effects of endogenous nitric oxide on pulmonary artery hypertension and lung injury induced by endotoxin

Mei Wan, Yi Ling Ling, Zhen Young Gu, Jun Luang Zhang, Shan Sheng Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Changes in mean artery pressure (MAP), pulmonary artery pressure (PAP) and nitric oxide (NO) contents in inflow and outflow pulmonary blood (IPB, OPB) were observed after endotoxin lipopolysacchride (LPS) was injected iv in rabbits. Changes of PAP and lung injury were also observed after inhibitor of NO synthesis L-NNA or inhibitor of inducible NO synthesis AG was pre-injected by vein. The results showed that MAP decreased significantly after LPS administration, and 0.5 - 2 h later PAP showed some increase (P < 0.05) being maximum at PAP (1 h) during which the content of NO in IPB was detectably decreased but NO in OPB did not. NO contents in OPB at 3 h and in IPB and OPB at 5 h increased significantly following LPS administration as compared with control. PAP correlated negatively with NO in IPB at the time before and 1 h after LPS injection, which did not exist at 3 and 5 h after LPS injection. After L-NNA pretreatment, when PAP elevated significantly, the MDA content in IPB and OPB also showed significant increase, while animal survival rate fell significantly. Light microscopic examination showed severe alveolar atelectasis, significant congestion and sequestration of leukocytes in lung tissue. When pretreated with AG, MAP elevated significantly in 3 - 5 h, PAP remained unchanged. The MDA content in blood was lower at 5 h in the LPS injected group with less pathological changes in lung tissue at 5 h compared with the LPS group. The above results suggested that there was pulmonary hypertension in the early stage after endotoxin administration. The decrease of NO content in IPB may be one of the mechanisms underlying pulmonary artery hypertension (PAH). NO seemed to alleviate PAH and lung injury at the early stage after endotoxin administration. When iNOS was induced at the later stage, NO contributed to lung injury caused by endotoxin.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalActa Physiologica Sinica
Volume51
Issue number1
StatePublished - Dec 1 1999
Externally publishedYes

Keywords

  • Endotoxin
  • Lung injury
  • Nitric oxide
  • Pulmonary artery hypertension
  • Rabbits

ASJC Scopus subject areas

  • Medicine(all)

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