Abstract
Burns have been associated with high levels of circulating pro-inflammatory cytokines which promote systemic inflammatory response syndrome (SIRS), immunosuppression and sepsis for which no effective treatment is currently available. Defensins, a family of cationic naturally occurring antimicrobial peptides, are considered important components of the innate immune system and enhance adaptive immunity. This study examines the effects of pro-inflammatory cytokines, interleukin-1β (IL-1β), gamma-interferon (IFNγ) and tumor necrosis factor-α (TNFα) on human β-defensin-2 (HBD-2) levels in cultured keratinocytes. We also examined the effects of heat shock at 42°C. The results demonstrate that only TNFα shows significant induction of HBD-2 but this induction was not sustained in the long-term. In addition, endogenous levels of defensin were significantly reduced by exposure to heat shock. The keratinocytes also responded to IL-1β by becoming hypertrophic. These results indicate that stress-related, pro-inflammatory cytokines can induce keratinocytes to synthesize HBD-2, while heat shock appears to reduce its production. These experiments give us further insight into the role of natural antimicrobial peptides under conditions of stress.
Original language | English (US) |
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Pages (from-to) | 329-333 |
Number of pages | 5 |
Journal | Burns |
Volume | 30 |
Issue number | 4 |
DOIs | |
State | Published - Jun 2004 |
Keywords
- Cytokines
- Defensin
- Fluorescent imaging
- Heat shock
- Keratinocytes
ASJC Scopus subject areas
- Surgery
- Emergency Medicine
- Critical Care and Intensive Care Medicine