Effects of commonly used topical medications on the rate of corneal reepithelialization following excimer laser keratectomy

C. W. Flowers, B. Seitz, C. Oliveira, U. Devgan, L. LaBree, Peter J McDonnell

Research output: Contribution to journalArticle

Abstract

Objective. To determine the effect of eight commonly used topical medications on the rate of corneal epithelial wound healing after excimer laser keratectomy. Methods. Six millimeter corneal epithelial defects were created in both eyes of 32 New Zealand white rabbits using a VISX 20/20 excimer laser. Rabbits were randomly assigned to one of eight treatment groups (Refresh Plus; diclofenac sodium, 0.1% (Voltaren); ketorolac, 0.5% (Acular); fluorometholone, 0.1% (FML); ofloxacin, 0.3% (Ocuflox); ciprofloxacin, 0.3% (Ciloxan); tobramycin, 0.3% (Tobrex); and gentamicin, 0.3% (Genoptic)). One eye received a topical medication six times a day and the other eye served as a control. Serial standardized color photography of the fluorescein-stained defects and computer-assisted planimetry of the projected photographs was used to quantitate epithelial defect areas, and the average daily rate of corneal epithelial wound healing was then calculated. Results. Compared to the other topical agents studied, fluorometholone 0.1% (4.67±1.14 mm2/day), and tobramycin 0.3% (5.70±1.15mm2/day), had the slowest mean wound healing rates (P <.001). The mean wound healing rates between fluorometholone and tobramycin were not statistically different. Similarly, the rates for all the other agents (8.34±1.08 to 9.93±1.14mm2/day) showed no significant difference. Comparison of wound healing rates between treated eyes and control eyes showed no significant difference for any of the topical medications studied. Conclusions. Fluorometholone, 0.1% and tobramycin, 0.3% significantly slow the rate of epithelial healing after excimer laser keratectomy in the rabbit compared to other commonly used topical medications. Our data also indicates that a significant cross-over effect occurs when the contralateral eye is used as a control.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Laser Corneal Surgery
Excimer Lasers
Fluorometholone
Tobramycin
Wound Healing
Diclofenac
Rabbits
Ketorolac Tromethamine
Ketorolac
Ofloxacin
Photography
Ciprofloxacin
Gentamicins
Fluorescein
Color

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effects of commonly used topical medications on the rate of corneal reepithelialization following excimer laser keratectomy. / Flowers, C. W.; Seitz, B.; Oliveira, C.; Devgan, U.; LaBree, L.; McDonnell, Peter J.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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title = "Effects of commonly used topical medications on the rate of corneal reepithelialization following excimer laser keratectomy",
abstract = "Objective. To determine the effect of eight commonly used topical medications on the rate of corneal epithelial wound healing after excimer laser keratectomy. Methods. Six millimeter corneal epithelial defects were created in both eyes of 32 New Zealand white rabbits using a VISX 20/20 excimer laser. Rabbits were randomly assigned to one of eight treatment groups (Refresh Plus; diclofenac sodium, 0.1{\%} (Voltaren); ketorolac, 0.5{\%} (Acular); fluorometholone, 0.1{\%} (FML); ofloxacin, 0.3{\%} (Ocuflox); ciprofloxacin, 0.3{\%} (Ciloxan); tobramycin, 0.3{\%} (Tobrex); and gentamicin, 0.3{\%} (Genoptic)). One eye received a topical medication six times a day and the other eye served as a control. Serial standardized color photography of the fluorescein-stained defects and computer-assisted planimetry of the projected photographs was used to quantitate epithelial defect areas, and the average daily rate of corneal epithelial wound healing was then calculated. Results. Compared to the other topical agents studied, fluorometholone 0.1{\%} (4.67±1.14 mm2/day), and tobramycin 0.3{\%} (5.70±1.15mm2/day), had the slowest mean wound healing rates (P <.001). The mean wound healing rates between fluorometholone and tobramycin were not statistically different. Similarly, the rates for all the other agents (8.34±1.08 to 9.93±1.14mm2/day) showed no significant difference. Comparison of wound healing rates between treated eyes and control eyes showed no significant difference for any of the topical medications studied. Conclusions. Fluorometholone, 0.1{\%} and tobramycin, 0.3{\%} significantly slow the rate of epithelial healing after excimer laser keratectomy in the rabbit compared to other commonly used topical medications. Our data also indicates that a significant cross-over effect occurs when the contralateral eye is used as a control.",
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AU - Seitz, B.

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AU - LaBree, L.

AU - McDonnell, Peter J

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N2 - Objective. To determine the effect of eight commonly used topical medications on the rate of corneal epithelial wound healing after excimer laser keratectomy. Methods. Six millimeter corneal epithelial defects were created in both eyes of 32 New Zealand white rabbits using a VISX 20/20 excimer laser. Rabbits were randomly assigned to one of eight treatment groups (Refresh Plus; diclofenac sodium, 0.1% (Voltaren); ketorolac, 0.5% (Acular); fluorometholone, 0.1% (FML); ofloxacin, 0.3% (Ocuflox); ciprofloxacin, 0.3% (Ciloxan); tobramycin, 0.3% (Tobrex); and gentamicin, 0.3% (Genoptic)). One eye received a topical medication six times a day and the other eye served as a control. Serial standardized color photography of the fluorescein-stained defects and computer-assisted planimetry of the projected photographs was used to quantitate epithelial defect areas, and the average daily rate of corneal epithelial wound healing was then calculated. Results. Compared to the other topical agents studied, fluorometholone 0.1% (4.67±1.14 mm2/day), and tobramycin 0.3% (5.70±1.15mm2/day), had the slowest mean wound healing rates (P <.001). The mean wound healing rates between fluorometholone and tobramycin were not statistically different. Similarly, the rates for all the other agents (8.34±1.08 to 9.93±1.14mm2/day) showed no significant difference. Comparison of wound healing rates between treated eyes and control eyes showed no significant difference for any of the topical medications studied. Conclusions. Fluorometholone, 0.1% and tobramycin, 0.3% significantly slow the rate of epithelial healing after excimer laser keratectomy in the rabbit compared to other commonly used topical medications. Our data also indicates that a significant cross-over effect occurs when the contralateral eye is used as a control.

AB - Objective. To determine the effect of eight commonly used topical medications on the rate of corneal epithelial wound healing after excimer laser keratectomy. Methods. Six millimeter corneal epithelial defects were created in both eyes of 32 New Zealand white rabbits using a VISX 20/20 excimer laser. Rabbits were randomly assigned to one of eight treatment groups (Refresh Plus; diclofenac sodium, 0.1% (Voltaren); ketorolac, 0.5% (Acular); fluorometholone, 0.1% (FML); ofloxacin, 0.3% (Ocuflox); ciprofloxacin, 0.3% (Ciloxan); tobramycin, 0.3% (Tobrex); and gentamicin, 0.3% (Genoptic)). One eye received a topical medication six times a day and the other eye served as a control. Serial standardized color photography of the fluorescein-stained defects and computer-assisted planimetry of the projected photographs was used to quantitate epithelial defect areas, and the average daily rate of corneal epithelial wound healing was then calculated. Results. Compared to the other topical agents studied, fluorometholone 0.1% (4.67±1.14 mm2/day), and tobramycin 0.3% (5.70±1.15mm2/day), had the slowest mean wound healing rates (P <.001). The mean wound healing rates between fluorometholone and tobramycin were not statistically different. Similarly, the rates for all the other agents (8.34±1.08 to 9.93±1.14mm2/day) showed no significant difference. Comparison of wound healing rates between treated eyes and control eyes showed no significant difference for any of the topical medications studied. Conclusions. Fluorometholone, 0.1% and tobramycin, 0.3% significantly slow the rate of epithelial healing after excimer laser keratectomy in the rabbit compared to other commonly used topical medications. Our data also indicates that a significant cross-over effect occurs when the contralateral eye is used as a control.

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