Effects of chronic intracameral injections of chondroitin sulfate on cat eyes

Pei F. Fei, Beatrice Y J T Yue, Mark O M Tso

Research output: Contribution to journalArticle

Abstract

The effect of chondroitin sulfate on the metabolism of trabecular meshwork was studied by repeatedly injecting a chondroitin sulfate solution into the anterior chamber of cat (experimental) eyes for 28-29 weeks. The aqueous humor of the opposite (control) eyes were similarly replaced with a phosphate-buffered saline-glucose solution. The intraocular pressure of the experimental eyes was increased 4-7 mm Hg above that of the control eyes for a prolonged period of time. In addition, collagen fibers, elastic fibers, and basement membrane-like material were moderately increased in the trabecular meshwork of experimental eyes. Three forms of thickened basement membranes of endothelial cells were observed. The compact multilaminated basement membrane was formed concentrically around some of the trabecular beams. The discontinuous, thin basement membrane was seen among bundles of coarse collagen in association with long spacing collagen fibers. Also, fine filaments were noted streaming from the plasma membrane and basement membrane of endothelial cells of angular aqueous veins. Chondroitinase ABC was able to remove ground substance in the elastic fiber of the trabecular meshwork. Biochemically, the chamber angle tissues from these eyes showed a higher synthetic activity of glycosaminoglycans and proteins. Chondroitin sulfate appears to have a direct effect on trabecular meshwork and can influence the physiologic function of these cells.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume222
Issue number1
DOIs
StatePublished - Oct 1984

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Chondroitin Sulfates
Trabecular Meshwork
Basement Membrane
Cats
Injections
Elastic Tissue
Collagen
Endothelial Cells
Chondroitin ABC Lyase
Aqueous Humor
Anterior Chamber
Glycosaminoglycans
Intraocular Pressure
Sodium Chloride
Veins
Phosphates
Cell Membrane
Glucose
Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effects of chronic intracameral injections of chondroitin sulfate on cat eyes. / Fei, Pei F.; Yue, Beatrice Y J T; Tso, Mark O M.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 222, No. 1, 10.1984, p. 1-8.

Research output: Contribution to journalArticle

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