Effects of charcoal-extracted serum as a growth medium supplement on in vitro development of mouse embryos

Santiago Padilla, Anita M. Howe, Jeffrey P. Boldt

Research output: Contribution to journalArticle

Abstract

Two-cell mouse embryos (N=952) were cultured in modified Ham's F-10 medium supplemented with 15% charcoal-extracted serum or 15% nonextracted serum from 17 patients. Each sample was assayed independently and all experiments run in duplicate. Ten nonextracted samples inhibited development to the blastocyst stage compared to controls in F-10 alone. Charcoal extraction significantly improved (P<0.05) development compared to nonextracted serum in eight of these samples. Seven samples supported development and no difference was noted between F-10 alone and extracted and nonextracted serum-supplemented media in this group. In conclusion, charcoal extraction significantly reversed the "embryotoxic" effects of some sera in the two-cell mouse embryo model.

Original languageEnglish (US)
Pages (from-to)286-289
Number of pages4
JournalJournal of in Vitro Fertilization and Embryo Transfer
Volume5
Issue number5
DOIs
Publication statusPublished - Oct 1 1988
Externally publishedYes

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Keywords

  • charcoal extraction
  • growth media
  • in vitro fertilization
  • mouse embryo
  • serum

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Embryology
  • Developmental Biology

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