Effects of centrifugating pressure on the function of preosteoblast OCT-1

Qin Bian, Zhu Yang, Shu Fen Liu, Xue Jun Cui, Qian Qian Liang, Yong Jian Zhao, Sheng Lu, Qi Shi, Yong Jun Wang

Research output: Contribution to journalArticlepeer-review

Abstract

ObjectiveTo investigate the feasibility of simulating gravity pressure and observe its effect on preosteoblast OCT-1 by centrifugation. MethodOCT-1 cells were cultured in 1% agarose gel with a final concentration of 2×107 cells per millilitre. The experiment was divided into two groups according to the duration: one day (1 d) and five days (5 d). Each group was further divided into three subgroups: 0 r/min (control), 200 r/min, and 500 r/min. The 200 r/min group and 500 r/min group were centrifuged for three hours once a day for one day or five days respectively. The control group was kept in the same environment without centrifugation. ResultsThe Col I positive staining was slightly strengthened in the 1d 200 r/min group while it was even more strengthened in the 1d 500 r/min group. Conversely, the positive staining was stronger in the 5d 200 r/min group than that in the 5d 500 r/min group. The markers related to osteoblast differentiation such as Alkaline phosphatise (ALP), Type I collagen α2 (Col1α2), Osteocalcin (OC) and runt-related transcription factor-2 (Runx2) mRNA expression were all up-regulated after centrifugation. ConclusionsCentrifugation is a practical method for cell pressure and plays a significant role in promoting the differentiation of preosteoblast OCT-1, which can be used as a new method to simulate the gravity.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalYiyong Shengwu Lixue/Journal of Medical Biomechanics
Volume25
Issue number6
StatePublished - Dec 1 2010
Externally publishedYes

Keywords

  • Cell culture
  • Cell pressure
  • Centrifugation
  • Gravity

ASJC Scopus subject areas

  • Biomedical Engineering

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