Effects of centrifugating pressure on the function of preosteoblast OCT-1

Qin Bian, Zhu Yang, Shu Fen Liu, Xue Jun Cui, Qian Qian Liang, Yong Jian Zhao, Sheng Lu, Qi Shi, Yong Jun Wang

Research output: Contribution to journalArticle

Abstract

ObjectiveTo investigate the feasibility of simulating gravity pressure and observe its effect on preosteoblast OCT-1 by centrifugation. MethodOCT-1 cells were cultured in 1% agarose gel with a final concentration of 2×10 7 cells per millilitre. The experiment was divided into two groups according to the duration: one day (1 d) and five days (5 d). Each group was further divided into three subgroups: 0 r/min (control), 200 r/min, and 500 r/min. The 200 r/min group and 500 r/min group were centrifuged for three hours once a day for one day or five days respectively. The control group was kept in the same environment without centrifugation. ResultsThe Col I positive staining was slightly strengthened in the 1d 200 r/min group while it was even more strengthened in the 1d 500 r/min group. Conversely, the positive staining was stronger in the 5d 200 r/min group than that in the 5d 500 r/min group. The markers related to osteoblast differentiation such as Alkaline phosphatise (ALP), Type I collagen α2 (Col1α2), Osteocalcin (OC) and runt-related transcription factor-2 (Runx2) mRNA expression were all up-regulated after centrifugation. ConclusionsCentrifugation is a practical method for cell pressure and plays a significant role in promoting the differentiation of preosteoblast OCT-1, which can be used as a new method to simulate the gravity.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalYiyong Shengwu Lixue/Journal of Medical Biomechanics
Volume25
Issue number6
Publication statusPublished - Dec 1 2010
Externally publishedYes

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Keywords

  • Cell culture
  • Cell pressure
  • Centrifugation
  • Gravity

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Bian, Q., Yang, Z., Liu, S. F., Cui, X. J., Liang, Q. Q., Zhao, Y. J., ... Wang, Y. J. (2010). Effects of centrifugating pressure on the function of preosteoblast OCT-1. Yiyong Shengwu Lixue/Journal of Medical Biomechanics, 25(6), 449-455.