Abstract
We investigated the effects of cataractogenesis on phospholipid (P-lipid) synthesis in sugar cataracts from galactosemic rats and in hereditary cataracts from 13/N guinea pigs. Cataractous lenses from rats fed a 50% galactose diet for 7 days were incubated 24 h with radiolabeled choline or ethanolamine and the P-lipids were extracted. The galactosemic cataracts synthesized twice as much phosphatidylcholine (PtdCho) as control rat lenses, and Phosphatidylethanolamine synthesis also was increased. Similar analysis of cataractous lenses from 3-week-old 13/N guinea pigs showed a 3-fold increase in PtdCho synthesis compared with control lenses. In all cases, the P-lipid precursor pool was lower in cataracts than in control lenses. The increased P-lipid synthesis in these cataracts may represent a membrane repair response to cataractogenic stress.
Original language | English (US) |
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Pages (from-to) | 7-12 |
Number of pages | 6 |
Journal | Ophthalmic Research |
Volume | 37 |
Issue number | 1 |
DOIs | |
State | Published - 2005 |
Keywords
- Galactosemia
- Phosphatidylcholine
- Phosphatidylethanolamine
- Phosphocholine
- Phosphoethanolamine
- Phospholipid synthesis
- Rat lens
- Strain 13/N guinea pig cataracts
- Sugar cataract
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience