Effects of cataractogenesis on the CDP-choline pathway: Increased phospholipid synthesis in lenses from galactosemic rats and 13/N guinea pigs

Howard M. Jernigan, Penny S. Blum, Ipsit Chakrabarti, Yin Su, J. Samuel Zigler

Research output: Contribution to journalArticle

Abstract

We investigated the effects of cataractogenesis on phospholipid (P-lipid) synthesis in sugar cataracts from galactosemic rats and in hereditary cataracts from 13/N guinea pigs. Cataractous lenses from rats fed a 50% galactose diet for 7 days were incubated 24 h with radiolabeled choline or ethanolamine and the P-lipids were extracted. The galactosemic cataracts synthesized twice as much phosphatidylcholine (PtdCho) as control rat lenses, and Phosphatidylethanolamine synthesis also was increased. Similar analysis of cataractous lenses from 3-week-old 13/N guinea pigs showed a 3-fold increase in PtdCho synthesis compared with control lenses. In all cases, the P-lipid precursor pool was lower in cataracts than in control lenses. The increased P-lipid synthesis in these cataracts may represent a membrane repair response to cataractogenic stress.

Original languageEnglish (US)
Pages (from-to)7-12
Number of pages6
JournalOphthalmic Research
Volume37
Issue number1
DOIs
StatePublished - May 25 2005

Keywords

  • Galactosemia
  • Phosphatidylcholine
  • Phosphatidylethanolamine
  • Phosphocholine
  • Phosphoethanolamine
  • Phospholipid synthesis
  • Rat lens
  • Strain 13/N guinea pig cataracts
  • Sugar cataract

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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