Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity

Esteban Mezey; James J. Potter; S. Mitchell Harmon; Panayiotis D. Tsitouras

doi:10.1016/0006-2952(80)90582-1

Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity

Esteban Mezey, James J. Potter, S. Mitchell Harmon, Panayiotis D. Tsitouras

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.) mmoles hr as compared with a value of 1.39 ± 0.125 mmoles hr in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD⁺/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.

Original language	English (US)
Pages (from-to)	3175-3180
Number of pages	6
Journal	Biochemical Pharmacology
Volume	29
Issue number	23
DOIs	https://doi.org/10.1016/0006-2952(80)90582-1
State	Published - Dec 1 1980

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(80)90582-1

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title = "Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity",

abstract = "The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.) mmoles hr as compared with a value of 1.39 ± 0.125 mmoles hr in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.",

author = "Esteban Mezey and Potter, {James J.} and Harmon, {S. Mitchell} and Tsitouras, {Panayiotis D.}",

note = "Funding Information: * This work was supported by Grant AA0626 from the United States Public Health Service. t Author to whom all correspondence should be addressed: Baltimore City Hospitals, 4940 Eastern Ave., Baltimore, MD 21224, U.S.A.",

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AU - Harmon, S. Mitchell

AU - Tsitouras, Panayiotis D.

N1 - Funding Information: * This work was supported by Grant AA0626 from the United States Public Health Service. t Author to whom all correspondence should be addressed: Baltimore City Hospitals, 4940 Eastern Ave., Baltimore, MD 21224, U.S.A.

PY - 1980/12/1

Y1 - 1980/12/1

N2 - The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.) mmoles hr as compared with a value of 1.39 ± 0.125 mmoles hr in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.

AB - The effects of castration and testosterone administration on the activity of liver alcohol dehydrogenase and on the rate of ethanol elimination were determined in male Sprague-Dawley rats. Castration increased liver alcohol dehydrogenase activity. The total liver activity in castrated animals was 2.37 ± 0.229 (S.E.) mmoles hr as compared with a value of 1.39 ± 0.125 mmoles hr in sham-operated controls (P < 0.01). Testosterone administration partially suppressed the enhanced activity of liver alcohol dehydrogenase produced by castration. By contrast, in control animals testosterone administration resulted in a small paradoxical increase in liver alcohol dehydrogenase. The increase in the enzyme activity in castrated animals was associated with a parallel increase in the rate of ethanol elimination. Castrated and control animals showed decreases in free cytosolic and mitochondrial NAD+/NADH ratios after ethanol administration. These observations suggest that testosterone (and probably other as yet unknown factors modified by castration) affects liver alcohol dehydrogenase activity, and that the total enzyme activity can be a principal limiting factor in ethanol elimination.

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Effects of castration and testosterone administration on rat liver alcohol dehydrogenase activity

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