Efeitos do pneumoperitônio com dióxido de carbono sobre a morfologia renal e hepática de ratos submetidos à colectomia segmentar e anastomose colônica

Translated title of the contribution: Effects of carbon dioxide pneumoperitoneum on hepatic and renal morphology of rats after segmental colectomy and colonic anastomosis

Igor Eduardo Caetano Farias, Pedro Henrique Alves de Morais, Leonardo de Castro Durães, Fabiana Pirani Carneiro, Paulo Gonçalves de Oliveira, João Batista de Sousa

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To evaluate the effects of increased intraperitoneal pressure caused by carbon dioxide pneumoperitoneum on the hepatic and renal morphology of rats. Methods: Fifty-four adult male rats were randomly divided into three groups (P, PP and C) after anesthesia: P - in 18 animals, pneumoperitoneum was established for 30 minutes immediately before laparotomy; PP - in 18 animals, pneumoperitoneum was established for 60 minutes divided into 30 immediately before laparotomy and 30 after abdominal closure;control group (C) - 18 animals underwent laparotomy without pneumoperitoneum induction. The pne umoperitoneum was maintained at a pressure of 5 mm Hg. Nine animals in each group were killed on the 3 rd and 7 th postoperative days, when kidney and liver samples were collected for morphological analysis. The liver specimens were stained with hematoxylin and eosin (HE), and the kidney specimens, with HE and von Kossa. Blinded examiners analyzed the slides. Results: No changes in renal morphology were found. Liver samples showed histological signs of degeneration in animals in the pneumoperitoneum groups killed on the 7 th postoperative day (p=0.029). Conclusion: The CO 2 pneumoperitoneum did not affect renal morphology but caused hydropic degeneration in the liver of animals killed on the 7 th postoperative day.

Translated title of the contributionEffects of carbon dioxide pneumoperitoneum on hepatic and renal morphology of rats after segmental colectomy and colonic anastomosis
Original languagePortuguese
Pages (from-to)279-284
Number of pages6
JournalActa cirurgica brasileira
Volume26
Issue number4
DOIs
StatePublished - Jul 2011

Keywords

  • Artificial
  • Carbon dioxide
  • Kidney
  • Laparoscopy
  • Liver
  • Pneumoperitoneum
  • Rats

ASJC Scopus subject areas

  • Surgery

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