Effects of Ca2+/calmodulin mediated signaling on spore germination and appressorium development in Colletotrichum falcatum went

M. Karunakaran, N. V. Nair, H. S. Rho, Yong H. Lee

Research output: Contribution to journalArticle

Abstract

Colletotrichum falcatum Went, the causal agent of red rot of sugarcane produces a specialized infection structure called appressorium, for penetrating the host. Environmental cues like surface hydrophobicity and hardness tend to break the dormancy of the conidia and initiate conidial germination. Conidial attachment is generally stronger on hydrophobic surfaces, while hydrophilic surfaces do not permit conidial attachment of C. falcatum. In vitro studies on conidial germination and appressorium development were made to examine whether the Ca2+/ cal-modulin dependent pathways are involved in appressorium formation in C. falcatum. Effects of calcium chelator (EGTA), calcium channel blocker (methoxy verampamil), calmodulin antagonists (chloropromazine, phenoxy benzamine and W-7) and phospholipase C inhibitor (neomycin) were also examined to see whether they can impair conidial germination and appressorium development. All these chemicals were found to inhibit conidial germination and or appressorium formation in C. falcatum. Chloropromazine and W-7 specifically inhibited appressorium formation at the μM level. Exogenous addition of Ca2+ was found to restore the, inhibition of conidial germination and appressorium development by EGTA. These results suggest that the Ca2+/calmodulin signal transduction pathway play an important role in the conidial morphogenesis and appressorium development in C. falcatum.

Original languageEnglish (US)
Pages (from-to)249-260
Number of pages12
JournalActa Phytopathologica et Entomologica Hungarica
Volume41
Issue number3-4
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Glomerella tucumanensis
appressoria
spore germination
calmodulin
calcium
germination
calcium channel blockers
neomycin
phospholipase C
chelating agents
hydrophobicity
in vitro studies
dormancy
conidia
morphogenesis
sugarcane
hardness
signal transduction
antagonists

Keywords

  • Appressoria
  • Colletotrichum falcatum
  • Infection structure
  • Red rot of sugarcane

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Effects of Ca2+/calmodulin mediated signaling on spore germination and appressorium development in Colletotrichum falcatum went. / Karunakaran, M.; Nair, N. V.; Rho, H. S.; Lee, Yong H.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 41, No. 3-4, 2006, p. 249-260.

Research output: Contribution to journalArticle

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