Effects of biphasic and monophasic electrical stimulation on mitochondrial dynamics, cell apoptosis, and cell proliferation

Maria R. Love, Jirapas Sripetchwandee, Siripong Palee, Siriporn C. Chattipakorn, Morton Maimon Mower, Nipon Chattipakorn

Research output: Contribution to journalArticle

Abstract

Currently, electrical stimulation (ES) is used to induce changes in various tissues and cellular processes, but its effects on mitochondrial dynamics and mechanisms are unknown. The aim of this study was to compare the effects of monophasic and biphasic, anodal, and cathodal ES on apoptosis, proliferation, and mitochondrial dynamics in neuroblastoma SH-SY5Y cells. Cells were cultured and treated with ES. Alamar blue assay was performed to measure cell proliferation. The proteins expression of apoptotic-related proteins Bcl-2 associated X (Bax), B cell lymphoma 2 (Bcl-2), optic-atrophy-1 (OPA1), mitofusin2 (Mfn2), phosphorylated dynamin-related protein 1 at serine 616 (p-DRP1), and total dynamin-related protein 1 (Total-DRP1) were also determined. The results showed that monophasic anodal and biphasic anodal/cathodal (Bi Anod) ES for 1 hr at 125 pulses per minute (2.0 Hz) produced the most significant increase in cell proliferation. In addition, monophasic anodal and Bi Anod ES treated cells displayed a significant increase in the levels of anti-apoptotic protein Bcl-2, whereas the Bax levels were not changed. Moreover, the levels of Mfn2 were increased in the cells treated by Bi Anod, and OPA1 was increased by monophasic anodal and Bi Anod ES, indicating increased mitochondrial fusion in these ES-treated cells. However, the levels of mitochondrial fission indicated by DRP1 remained unchanged compared with non-stimulated cells. These findings were confirmed through visualization of mitochondria using Mitotracker Deep Red, demonstrating that monophasic anodal and Bi Anod ES could induce pro-survival effects in SH-SY5Y cells through increasing cell proliferation and mitochondrial fusion. Future research is needed to validate these findings for the clinical application of monophasic anodal and Bi Anod ES.

Original languageEnglish (US)
JournalJournal of Cellular Physiology
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Fingerprint

Mitochondrial Dynamics
Cell proliferation
Electric Stimulation
Dynamins
Cells
Cell Proliferation
Apoptosis
Optics
Fusion reactions
Apoptosis Regulatory Proteins
Proteins
Mitochondria
B-Cell Lymphoma
Autosomal Dominant Optic Atrophy
Serine
Assays
Visualization
Tissue
Cell Fusion
Neuroblastoma

Keywords

  • apoptosis
  • biphasic stimulation
  • cell proliferation
  • electrotherapy
  • mitochondrial dynamics
  • monophasic stimulation

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Effects of biphasic and monophasic electrical stimulation on mitochondrial dynamics, cell apoptosis, and cell proliferation. / Love, Maria R.; Sripetchwandee, Jirapas; Palee, Siripong; Chattipakorn, Siriporn C.; Mower, Morton Maimon; Chattipakorn, Nipon.

In: Journal of Cellular Physiology, 01.01.2018.

Research output: Contribution to journalArticle

Love, Maria R. ; Sripetchwandee, Jirapas ; Palee, Siripong ; Chattipakorn, Siriporn C. ; Mower, Morton Maimon ; Chattipakorn, Nipon. / Effects of biphasic and monophasic electrical stimulation on mitochondrial dynamics, cell apoptosis, and cell proliferation. In: Journal of Cellular Physiology. 2018.
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AU - Mower, Morton Maimon

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