Effects of anesthesia and species on the uptake or binding of radioligands in vivo in the Göttingen minipig

Aage K O Alstrup, Anne M. Landau, James E. Holden, Steen Jakobsen, Anna C. Schacht, Helene Audrain, Gregers Wegener, Axel K. Hansen, Albert Gjedde, Doris J. Doudet

Research output: Contribution to journalArticle

Abstract

Progress in neuroscience research often involves animals, as no adequate alternatives exist to animal models of living systems. However, both the physiological characteristics of the species used and the effects of anesthesia raise questions of common concern. Here, we demonstrate the confounding influences of these effects on tracer binding in positron emission tomography (PET). We determined the effects of two routinely used anesthetics (isoflurane and propofol) on the binding of two tracers of monoamine function, [ C 11 ]SCH23390, a tracer of the dopamine D1 and D5 receptors, and the alpha2-adrenoceptor antagonist, [ C 11 ]yohimbine, in Göttingen minipigs. The kinetics of SCH23390 in the pigs differed from those of our earlier studies in primates. With two different graphical analyses of uptake of SCH23390, the initial clearance values of this tracer were higher with isoflurane than with propofol anesthesia, indicative of differences in blood flow, whereas no significant differences were observed for the volumes of distribution of yohimbine. The study underscores the importance of differences of anesthesia and species when the properties of radioligands are evaluated under different circumstances that may affect blood flow and tracer uptake. These differences must be considered in the choice of a particular animal species and mode of anesthesia for a particular application.

Original languageEnglish (US)
Article number808713
JournalBioMed Research International
Volume2013
DOIs
StatePublished - 2013
Externally publishedYes

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Miniature Swine
Animals
Yohimbine
Anesthesia
Isoflurane
Propofol
Dopamine D5 Receptors
Blood
Dopamine D1 Receptors
Positron emission tomography
Adrenergic Receptors
Anesthetics
Neurosciences
Positron-Emission Tomography
Primates
Kinetics
Swine
Animal Models
SCH 23390
Research

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Alstrup, A. K. O., Landau, A. M., Holden, J. E., Jakobsen, S., Schacht, A. C., Audrain, H., ... Doudet, D. J. (2013). Effects of anesthesia and species on the uptake or binding of radioligands in vivo in the Göttingen minipig. BioMed Research International, 2013, [808713]. https://doi.org/10.1155/2013/808713

Effects of anesthesia and species on the uptake or binding of radioligands in vivo in the Göttingen minipig. / Alstrup, Aage K O; Landau, Anne M.; Holden, James E.; Jakobsen, Steen; Schacht, Anna C.; Audrain, Helene; Wegener, Gregers; Hansen, Axel K.; Gjedde, Albert; Doudet, Doris J.

In: BioMed Research International, Vol. 2013, 808713, 2013.

Research output: Contribution to journalArticle

Alstrup, AKO, Landau, AM, Holden, JE, Jakobsen, S, Schacht, AC, Audrain, H, Wegener, G, Hansen, AK, Gjedde, A & Doudet, DJ 2013, 'Effects of anesthesia and species on the uptake or binding of radioligands in vivo in the Göttingen minipig', BioMed Research International, vol. 2013, 808713. https://doi.org/10.1155/2013/808713
Alstrup, Aage K O ; Landau, Anne M. ; Holden, James E. ; Jakobsen, Steen ; Schacht, Anna C. ; Audrain, Helene ; Wegener, Gregers ; Hansen, Axel K. ; Gjedde, Albert ; Doudet, Doris J. / Effects of anesthesia and species on the uptake or binding of radioligands in vivo in the Göttingen minipig. In: BioMed Research International. 2013 ; Vol. 2013.
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