Effects of airway distension on leukocyte recruitment in the mouse tracheal microvasculature

Elizabeth M. Wagner, John Jenkins

Research output: Contribution to journalArticlepeer-review


We have shown previously that excessive distention of the rat trachea during mechanical ventilation results in enhanced leukocyte recruitment to the airway (Lim LH and Wagner EM. Am J Respir Crit Care Med 168:1068 -1074, 2003). The objectives of this study were to develop a mouse model of positive end-expiratory pressure (PEEP)-induced leukocyte recruitment to the airway and begin to pursue molecular mechanisms that may contribute to the in vivo observation of increased leukocyte adhesion after PEEP exposure. We studied C57BL/6 wild-type mice and mice deficient in P-selectin or intercellular adhesion molecule-1 (ICAM-1) exposed to intermittent PEEP (8 cmH2O) applied five times for a 1-min duration, at 10-min intervals. After the imposed ventilatory stress, during normal ventilation (0.2 ml/breath, no PEEP), leukocyte adhesion in tracheal postcapillary venules was determined using intravital microscopy. PEEP induced a time-dependent increase in leukocyte adhesion that was significantly increased between 0 and 60 min (P < 0.01). Furthermore, PEEP-induced leukocyte adhesion at 60 min was ablated in P-selectin- and ICAM-1-deficient mice. These findings demonstrate the essential nature of both P-selectin and ICAM-1 within airway postcapillary venular endothelium for leukocyte recruitment after airway distension.

Original languageEnglish (US)
Pages (from-to)1528-1534
Number of pages7
JournalJournal of applied physiology
Issue number4
StatePublished - Apr 2007


  • Endothelial activation
  • Intravital microscopy
  • Mechanical stress
  • Positive end-expiratory pressure

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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