Effects of age on pilocarpine-induced c-fos expression in rat hippocampus and cortex

D. J. Bucci, D. L. Rosen, M. Gallagher

Research output: Contribution to journalArticle

Abstract

The effect of age on pilocarpine-induced expression of the immediate- early gene c-fos was examined in the hippocampus and cortex of Long-Evans rats. Rats were treated with either pilocarpine (25 mg/kg) or saline, and sacrificed 90 min. following injection. The level of c-fos mRNA and Fos-like protein expression was determined using in situ hybridization histochemistry, and immunocytochemistry, respectively. In saline-treated animals, comparable levels of c-fos mRNA and Fos-like protein were observed in the hippocampus and cortical regions of young (6 month) and aged (24-26 months) rats. The expression of Fos-like protein following pilocarpine treatment was increased, however, in frontal, retrosplenial, and cingulate cortex of aged compared to young rats. In frontal and retrosplenial cortex, the changes in Fos-like protein were accompanied by changes in c-fos mRNA expression. In contrast, no age difference was detected in the hippocampus or parietal cortex of pilocarpine-treated rats. These regionally-specific age differences in response to pilocarpine administration suggest that mechanisms localized to those areas play an important role in determining the response to cholinergic stimulation mediated through post-synaptic muscarinic receptors.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalNeurobiology of Aging
Volume19
Issue number3
DOIs
StatePublished - May 1 1998

Keywords

  • Acetylcholine
  • Aging
  • Cortex
  • Hippocampus
  • Immediate-early genes
  • Muscarinic receptors
  • Pilocarpine
  • Rat
  • c-fos

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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