TY - JOUR
T1 - Effects of age on muscarinic agonist-induced contraction and IP accumulation in airway smooth muscle
AU - Wills-Karp, Marsha
N1 - Funding Information:
The author wishes to thank Dr. Nicholas Flavahan for critical review of the manuscript and Ms. Cindy Rogers for excellent secretarial assistance. This investigation was supported by research grants from the National Heart, Blood and Lung Institute, U.S.A. HL-43312 and HI-10342.
PY - 1991
Y1 - 1991
N2 - The effects of age on carbachol-stimulated force development and [3H]inositol phosphate production was studied in tracheal rings from guinea pigs aged 1 month and 25 months of age. The pD2 for the contractile response to carbochol was significantly reduced in tracheal tissues from old animals as compared to that of the young tissues (6.49±0.04, 7.09±0.04, n=12), respectively. In contrast, inositol phosphate formation was not altered with increasing age when stimulated by carbachol or NaF, a direct activator of G proteins. Carbachol-induced inositol phosphate accumulation was inhibited by treatment with 1ug/ml pertussis toxin, suggesting that IP1 accumulation is coupled to a pertussis-toxin-sensitive protein. The pD2 values for contraction (7.09±0.09, 6.49±0.04) were significantly different from the pD2 values for IP1 accumulation (4.72±0.14, 5.10±0.18) in both young and old tissues, respectively. These data suggest that IP1 accumulation is not responsible for the decreased contractile ability in tracheal smooth muscle during aging.
AB - The effects of age on carbachol-stimulated force development and [3H]inositol phosphate production was studied in tracheal rings from guinea pigs aged 1 month and 25 months of age. The pD2 for the contractile response to carbochol was significantly reduced in tracheal tissues from old animals as compared to that of the young tissues (6.49±0.04, 7.09±0.04, n=12), respectively. In contrast, inositol phosphate formation was not altered with increasing age when stimulated by carbachol or NaF, a direct activator of G proteins. Carbachol-induced inositol phosphate accumulation was inhibited by treatment with 1ug/ml pertussis toxin, suggesting that IP1 accumulation is coupled to a pertussis-toxin-sensitive protein. The pD2 values for contraction (7.09±0.09, 6.49±0.04) were significantly different from the pD2 values for IP1 accumulation (4.72±0.14, 5.10±0.18) in both young and old tissues, respectively. These data suggest that IP1 accumulation is not responsible for the decreased contractile ability in tracheal smooth muscle during aging.
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U2 - 10.1016/0024-3205(91)90305-U
DO - 10.1016/0024-3205(91)90305-U
M3 - Article
C2 - 1653887
AN - SCOPUS:0025879290
SN - 0024-3205
VL - 49
SP - 1039
EP - 1045
JO - Life Sciences
JF - Life Sciences
IS - 14
ER -