Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium

Giovanni Gambassi, Richard G. Hansford, Steven J. Sollott, Barbara A. Hogue, Edward Lakatta, Maurizio C. Capogrossi

Research output: Contribution to journalArticle

Abstract

Acidosis increases resting cytosolic [Ca2+], (Cai) of myocardial prepa-rations; however, neither the Ca2+ sources for the increase in Cai nor the effect of acidosis on mitochondrial free [Ca2+], (Cam) have been characterized. In this study cytosolic pH (pHi) was monitored in adult rat left ventricular myocytes loaded with the acetoxymethyl ester (AM form) of SNARF-1. A stable decrease in the pHi of 0.52 ± 0.05 U (n = 16) was obtained by switching from a bicarbonate buffer equilibrated with 5% CO2 to a buffer equilibrated with 20% CO2. Electrical stimulation at either 0.5 or 1.5 Hz had no effect on pHi in 5% CO2, nor did it affect the magnitude of pHi decrease in response to hypercarbic acidosis. Cai was measured in myocytes loaded with indo-1/free acid and Cam was monitored in cells loaded with indo-1/AM after quenching cytosolic indo-1 fluorescence with MnCl2. In quiescent intact myocytes bathed in 1.5 mM [Ca2+], hypercarbia increased Cai from 130 ± 5 to 221 ± 13 nM. However, when acidosis was effected in electrically stimulated myocytes, diastolic Cai increased more than resting Cai in quiescent myocytes, and during pacing at 1.5 Hz diastolic Cai was higher (285 ± 17 nM) than at 0.5 Hz (245 ± 18 nM; P <0.05). The magnitude of Cai increase in quiescent myocytes was not affected either by sarcoplasmic reticulum (SR) Ca2+ depletion with ryanodine or by SR Ca2+ depletion and concomitant superfusion with a Ca2+-free buffer. In unstimulated intact myocytes hypercarbia increased Cam from 95 ± 12 to 147 ± 19 nM and this response was not modified either by ryanodine and a Ca2+-free buffer or by 50 μM ruthenium red in order to block the mitochondrial uniporter. In mitochondrial suspensions loaded either with BCECF/AM or indo-1/ AM, acidosis produced by lactic acid addition decreased both intra- and extramitochondrial pH and increased Cam. Studies of mitochondrial suspensions bathed in indo-1/free acid - containing solution showed an increase in extramitochondrial Ca2+ after the addition of lactic acid. Thus, in quiescent myocytes, cytoplasmic and intramitochondrial buffers, rather than transsarcolemmal Ca2+ influx or SR Ca2+ release, are the likely Ca2+ sources for the increase in Cai and Cam, respectively; additionally, Ca2+ efflux from the mitochondria may contribute to the raise in Cai. In contrast, in response to acidosis, diastolic Cai in electrically stimulated myocytes increases more than resting Cai in quiescent cells; this suggests that during pacing, net cell Ca2+ gain contributes to enhance diastolic Cai.

Original languageEnglish (US)
Pages (from-to)575-597
Number of pages23
JournalJournal of General Physiology
Volume102
Issue number3
StatePublished - Sep 1993
Externally publishedYes

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Acidosis
Muscle Cells
Myocardium
Buffers
Sarcoplasmic Reticulum
Ryanodine
Hypercapnia
Lactic Acid
Suspensions
Ruthenium Red
Acids
Bicarbonates
Electric Stimulation
Mitochondria
Esters
Fluorescence

ASJC Scopus subject areas

  • Physiology

Cite this

Gambassi, G., Hansford, R. G., Sollott, S. J., Hogue, B. A., Lakatta, E., & Capogrossi, M. C. (1993). Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium. Journal of General Physiology, 102(3), 575-597.

Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium. / Gambassi, Giovanni; Hansford, Richard G.; Sollott, Steven J.; Hogue, Barbara A.; Lakatta, Edward; Capogrossi, Maurizio C.

In: Journal of General Physiology, Vol. 102, No. 3, 09.1993, p. 575-597.

Research output: Contribution to journalArticle

Gambassi, G, Hansford, RG, Sollott, SJ, Hogue, BA, Lakatta, E & Capogrossi, MC 1993, 'Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium', Journal of General Physiology, vol. 102, no. 3, pp. 575-597.
Gambassi G, Hansford RG, Sollott SJ, Hogue BA, Lakatta E, Capogrossi MC. Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium. Journal of General Physiology. 1993 Sep;102(3):575-597.
Gambassi, Giovanni ; Hansford, Richard G. ; Sollott, Steven J. ; Hogue, Barbara A. ; Lakatta, Edward ; Capogrossi, Maurizio C. / Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium. In: Journal of General Physiology. 1993 ; Vol. 102, No. 3. pp. 575-597.
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T1 - Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium

AU - Gambassi, Giovanni

AU - Hansford, Richard G.

AU - Sollott, Steven J.

AU - Hogue, Barbara A.

AU - Lakatta, Edward

AU - Capogrossi, Maurizio C.

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N2 - Acidosis increases resting cytosolic [Ca2+], (Cai) of myocardial prepa-rations; however, neither the Ca2+ sources for the increase in Cai nor the effect of acidosis on mitochondrial free [Ca2+], (Cam) have been characterized. In this study cytosolic pH (pHi) was monitored in adult rat left ventricular myocytes loaded with the acetoxymethyl ester (AM form) of SNARF-1. A stable decrease in the pHi of 0.52 ± 0.05 U (n = 16) was obtained by switching from a bicarbonate buffer equilibrated with 5% CO2 to a buffer equilibrated with 20% CO2. Electrical stimulation at either 0.5 or 1.5 Hz had no effect on pHi in 5% CO2, nor did it affect the magnitude of pHi decrease in response to hypercarbic acidosis. Cai was measured in myocytes loaded with indo-1/free acid and Cam was monitored in cells loaded with indo-1/AM after quenching cytosolic indo-1 fluorescence with MnCl2. In quiescent intact myocytes bathed in 1.5 mM [Ca2+], hypercarbia increased Cai from 130 ± 5 to 221 ± 13 nM. However, when acidosis was effected in electrically stimulated myocytes, diastolic Cai increased more than resting Cai in quiescent myocytes, and during pacing at 1.5 Hz diastolic Cai was higher (285 ± 17 nM) than at 0.5 Hz (245 ± 18 nM; P <0.05). The magnitude of Cai increase in quiescent myocytes was not affected either by sarcoplasmic reticulum (SR) Ca2+ depletion with ryanodine or by SR Ca2+ depletion and concomitant superfusion with a Ca2+-free buffer. In unstimulated intact myocytes hypercarbia increased Cam from 95 ± 12 to 147 ± 19 nM and this response was not modified either by ryanodine and a Ca2+-free buffer or by 50 μM ruthenium red in order to block the mitochondrial uniporter. In mitochondrial suspensions loaded either with BCECF/AM or indo-1/ AM, acidosis produced by lactic acid addition decreased both intra- and extramitochondrial pH and increased Cam. Studies of mitochondrial suspensions bathed in indo-1/free acid - containing solution showed an increase in extramitochondrial Ca2+ after the addition of lactic acid. Thus, in quiescent myocytes, cytoplasmic and intramitochondrial buffers, rather than transsarcolemmal Ca2+ influx or SR Ca2+ release, are the likely Ca2+ sources for the increase in Cai and Cam, respectively; additionally, Ca2+ efflux from the mitochondria may contribute to the raise in Cai. In contrast, in response to acidosis, diastolic Cai in electrically stimulated myocytes increases more than resting Cai in quiescent cells; this suggests that during pacing, net cell Ca2+ gain contributes to enhance diastolic Cai.

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