Effects of acetylsalicylic acid (aspirin) and naproxen sodium (naproxen) on ovulation, prostaglandin, and progesterone production in the rabbit

V. Zanagnolo, A. M. Dharmarajan, K. Endo, Edward E Wallach

Research output: Contribution to journalArticle

Abstract

Objective: To determine the effects of acetylsalicylic acid (aspirin) and naproxen sodium (naproxen) on ovulation, ovarian prostaglandins (PG), and P production in the rabbit via in vivo and in vitro studies. Design: Aspirin and naproxen were administered IV 6.5 and 7 hours, respectively, after hCG administration to New Zealand White adult female rabbits. Laparotomy was performed 24 hours after hCG administration. For in vitro experiments, control animals underwent laparotomy 6.5 (aspirin) and 7 hours (naproxen) after hCG administration. The treated animal received aspirin and naproxen; laparotomy was performed 1 hour later. One ovary was perfused for 6 hours with aspirin or naproxen whereas the contralateral ovary served as a control and was perfused with control medium (M199; GIBCO, Grand Island, New York). Perfusate samples were collected at 1-hour intervals for PG and P determination. Setting: A conventional laboratory setting. Interventions: In vivo experiments used IV administration of 100 mg/kg aspirin and 10 and 50 mg/kg naproxen. In vitro perfusion was also carried out with 100 μg/mL aspirin and 10 and 50 μg/mL naproxen added to the perfusate. Main Outcome Measures: Ovulatory efficiency (no. of ovulations/no mature follicles) and ovarian vein PG and P concentration were determined. Results: Ovulatory efficiency was 88% for control, 41% for in vivo aspirin-treated, and 40% (10 mg/kg) and 0% (50 mg/kg) for naproxen-treated rabbits. Aspirin and naproxen were associated with decreased ovulatory efficiency when administered in vitro to both in vivo control and in vivo treated ovaries (control-medium = 70%; control-aspirin = 14%; aspirin-medium = 34%; aspirin-aspirin = 0%; control-naproxen = 25%; naproxen-medium = 38%; naproxen = 0% with 10 μg/mL, and control-naproxen = 13%; naproxen-medium = 0%; naproxen = 0% with 50 μg/mL). Prostaglandin F2α was undetectable in the perfusate of those ovaries perfused either with aspirin or naproxen. Ovarian venous concentration of P in the perfusate was similar in all groups. Conclusions: Aspirin and naproxen significantly reduced ovulatory efficiency and PG production both in vivo and in vitro in hCG-treated rabbits. A critical period of 6.5 and 7 hours after hCG administration was established.

Original languageEnglish (US)
Pages (from-to)1036-1043
Number of pages8
JournalFertility and Sterility
Volume65
Issue number5
StatePublished - 1996

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Naproxen
Ovulation
Aspirin
Prostaglandins
Progesterone
Sodium
Rabbits
Ovary
Laparotomy
Dinoprost
Ovarian Follicle

Keywords

  • Aspirin
  • inhibition of ovulation
  • naproxen
  • prostaglandin

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Effects of acetylsalicylic acid (aspirin) and naproxen sodium (naproxen) on ovulation, prostaglandin, and progesterone production in the rabbit. / Zanagnolo, V.; Dharmarajan, A. M.; Endo, K.; Wallach, Edward E.

In: Fertility and Sterility, Vol. 65, No. 5, 1996, p. 1036-1043.

Research output: Contribution to journalArticle

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abstract = "Objective: To determine the effects of acetylsalicylic acid (aspirin) and naproxen sodium (naproxen) on ovulation, ovarian prostaglandins (PG), and P production in the rabbit via in vivo and in vitro studies. Design: Aspirin and naproxen were administered IV 6.5 and 7 hours, respectively, after hCG administration to New Zealand White adult female rabbits. Laparotomy was performed 24 hours after hCG administration. For in vitro experiments, control animals underwent laparotomy 6.5 (aspirin) and 7 hours (naproxen) after hCG administration. The treated animal received aspirin and naproxen; laparotomy was performed 1 hour later. One ovary was perfused for 6 hours with aspirin or naproxen whereas the contralateral ovary served as a control and was perfused with control medium (M199; GIBCO, Grand Island, New York). Perfusate samples were collected at 1-hour intervals for PG and P determination. Setting: A conventional laboratory setting. Interventions: In vivo experiments used IV administration of 100 mg/kg aspirin and 10 and 50 mg/kg naproxen. In vitro perfusion was also carried out with 100 μg/mL aspirin and 10 and 50 μg/mL naproxen added to the perfusate. Main Outcome Measures: Ovulatory efficiency (no. of ovulations/no mature follicles) and ovarian vein PG and P concentration were determined. Results: Ovulatory efficiency was 88{\%} for control, 41{\%} for in vivo aspirin-treated, and 40{\%} (10 mg/kg) and 0{\%} (50 mg/kg) for naproxen-treated rabbits. Aspirin and naproxen were associated with decreased ovulatory efficiency when administered in vitro to both in vivo control and in vivo treated ovaries (control-medium = 70{\%}; control-aspirin = 14{\%}; aspirin-medium = 34{\%}; aspirin-aspirin = 0{\%}; control-naproxen = 25{\%}; naproxen-medium = 38{\%}; naproxen = 0{\%} with 10 μg/mL, and control-naproxen = 13{\%}; naproxen-medium = 0{\%}; naproxen = 0{\%} with 50 μg/mL). Prostaglandin F2α was undetectable in the perfusate of those ovaries perfused either with aspirin or naproxen. Ovarian venous concentration of P in the perfusate was similar in all groups. Conclusions: Aspirin and naproxen significantly reduced ovulatory efficiency and PG production both in vivo and in vitro in hCG-treated rabbits. A critical period of 6.5 and 7 hours after hCG administration was established.",
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AU - Zanagnolo, V.

AU - Dharmarajan, A. M.

AU - Endo, K.

AU - Wallach, Edward E

PY - 1996

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N2 - Objective: To determine the effects of acetylsalicylic acid (aspirin) and naproxen sodium (naproxen) on ovulation, ovarian prostaglandins (PG), and P production in the rabbit via in vivo and in vitro studies. Design: Aspirin and naproxen were administered IV 6.5 and 7 hours, respectively, after hCG administration to New Zealand White adult female rabbits. Laparotomy was performed 24 hours after hCG administration. For in vitro experiments, control animals underwent laparotomy 6.5 (aspirin) and 7 hours (naproxen) after hCG administration. The treated animal received aspirin and naproxen; laparotomy was performed 1 hour later. One ovary was perfused for 6 hours with aspirin or naproxen whereas the contralateral ovary served as a control and was perfused with control medium (M199; GIBCO, Grand Island, New York). Perfusate samples were collected at 1-hour intervals for PG and P determination. Setting: A conventional laboratory setting. Interventions: In vivo experiments used IV administration of 100 mg/kg aspirin and 10 and 50 mg/kg naproxen. In vitro perfusion was also carried out with 100 μg/mL aspirin and 10 and 50 μg/mL naproxen added to the perfusate. Main Outcome Measures: Ovulatory efficiency (no. of ovulations/no mature follicles) and ovarian vein PG and P concentration were determined. Results: Ovulatory efficiency was 88% for control, 41% for in vivo aspirin-treated, and 40% (10 mg/kg) and 0% (50 mg/kg) for naproxen-treated rabbits. Aspirin and naproxen were associated with decreased ovulatory efficiency when administered in vitro to both in vivo control and in vivo treated ovaries (control-medium = 70%; control-aspirin = 14%; aspirin-medium = 34%; aspirin-aspirin = 0%; control-naproxen = 25%; naproxen-medium = 38%; naproxen = 0% with 10 μg/mL, and control-naproxen = 13%; naproxen-medium = 0%; naproxen = 0% with 50 μg/mL). Prostaglandin F2α was undetectable in the perfusate of those ovaries perfused either with aspirin or naproxen. Ovarian venous concentration of P in the perfusate was similar in all groups. Conclusions: Aspirin and naproxen significantly reduced ovulatory efficiency and PG production both in vivo and in vitro in hCG-treated rabbits. A critical period of 6.5 and 7 hours after hCG administration was established.

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