Effects of 2 deoxyglucose, glucosamine, and mannose on cell fusion and the glycoproteins of herpes simplex virus

R. W. Knowles, S. Person

Research output: Contribution to journalArticlepeer-review

Abstract

2 Deoxyglucose and glucosamine were found to inhibit cell fusion caused by a syncytial mutant of herpes simplex virus and to inhibit the glycosylation of viral glycoproteins in the infected cells. The inhibition of fusion and the inhibition of glycosylation caused by 2 deoxyglucose were substantially prevented when mannose was also present during infection. When glycosylation was inhibited, 3 new bands were found in the major glycoprotein region on sodium dodecyl sulfate polyacrylamide gels. These bands may be precursors to the normal glycoproteins. The correlation between fusion and glycosylation in the presence of 2 deoxyglucose, glucosamine, and mannose suggests that the cells cannot fuse if their glycoproteins have a considerably reduced carbohydrate content.

Original languageEnglish (US)
Pages (from-to)644-651
Number of pages8
JournalJournal of virology
Volume18
Issue number2
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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