Effects of 2-amino-4,6-dinitrotoluene on p53 tumor suppressor gene expression

Hirendra Banerjee, Zack Hawkins, Sisir Dutta, Duane Smoot

Research output: Contribution to journalArticle

Abstract

2-Amino-4,6-dinitrotoluene (2-Am-DNT) and its isomers are the most common metabolites of 2,4,6-trinitrotoluene (TNT). It is desirable to know the toxicity of this metabolite particularly because of its role in carcinogenicity and because it could contaminate drinking water. We used MCF-7 human breast cancer cells which have p53 tumor suppressor gene in wild type form in both the loci. Immunoblotting with p53 antibody showed enhanced p53 level in treated cells compared to untreated control cells. Similarly, p53 DNA-protein binding assays (gel-shift) showed accumulation of p53 protein in treated cells. This is the first report which shows p53 accumulation in 2-Am-DNT treated cells providing evidence of potential carcinogenic effects of 2-Am-DNT.

Original languageEnglish (US)
Pages (from-to)387-389
Number of pages3
JournalMolecular and Cellular Biochemistry
Volume252
Issue number1-2
DOIs
StatePublished - Oct 1 2003

Keywords

  • 2-Am-DNT
  • MCF7 cells
  • Tumor suppressor
  • p53 protein

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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