Effects of 10% formalin fixation on fixed lung volume and lung tissue shrinkage . A comparison of eleven laboratory species

H. Lum, W. Mitzner

Research output: Contribution to journalArticle

Abstract

This species comparative study examined tissue shrinkage from a known physiologic lung volume through to the processed histologic section. Eleven mammalian species with body weights that spanned 3 orders of magnitude were studied. Air pressure-volume curves were determined to obtain total lung capacity (TLC) at 30 cmH2O. The lungs were then fixed by airway filling at 25 cmH2O pressure, and a displacement fixed lung volume was determined. Linear dimensions were systematically measured on fixed tissue blocks, embedded tissue blocks, and stained sections. Results indicated that the ratio of fixed lung volume to TLC ranged between 0.6 and 2.0. The corresponding ratios for linear dimensions ranged between 0.8 and 1.3 for all species. Histologic processing caused further shrinkage; the ratios of linear dimensions measured after and to those measured before processing ranged between 0.6 and 0.7. Thus, the degree of fixed lung volume achieved relative to TLC varies considerably more among species than does the histologic shrinkage caused by processing. We conclude that measurement of these changes in lung dimension caused by fixation and histologic processing in the different species is essential, particularly in quantitative interspecies physiologic studies.

Original languageEnglish (US)
Pages (from-to)1078-1083
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume132
Issue number5
StatePublished - Dec 1 1985

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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