Effects and mechanisms of auricular vagus nerve stimulation on high-fat-diet-induced obese rats

Li Han, Zhang Jian-Bin, Xu Chen, Tang Qing-Qing, Shen Wei-Xing, Zhou Jing-Zhu, Jiande Chen, Wang Yin-Ping

Research output: Contribution to journalArticle

Abstract

Objective: Obesity is a major public health problem. Regulating food intake and promoting metabolism of fat are two important options for treating obesity. Auricular vagus nerve stimulation (AVNS) is considered as an alternative approach to vagal nerve stimulation. The aim of this study was to investigate the effects of AVNS and its mechanisms on obesity in obese rats. Methods: Male Sprague-Dawley rats were fed either a high-fat diet (HFD) or a normal diet for 8 wk. Qualified HFD rats were randomly divided into three groups: the HFD group, the AVNS group, and the sham group for 6 wk treatment. Body weight and daily energy intake were recorded weekly. The rats were sacrificed for measurement of weight of bilateral perirenal, epididymal white adipose tissue (WAT), dorsal brown adipose tissue (BAT), and gastric emptying. Serum cholecystokinin (CCK), peptide YY3 to 36 (PYY3-36) and norepinephrine (NE) were assayed by enzyme-linked immunosorbent assay. Real-time quantitative polymerase chain reaction was used to assess the mRNA expressions of CCK subtype receptor a (CCKa) in the antrum, PYY3-36 receptor in the distal ileum, β3-adrenoceptor, and uncoupling protein gene 1 (UCP1) in the BAT. Results: Compared with HFD group, AVNS significantly reduced body weight and epididymal WAT and increased BAT weight, serum NE, mRNA expressions of β3-adrenoceptors, and UCP1 of the BAT, but had no effect on daily energy intake, perirenal WAT weight, gastric emptying, serum levels of CCK and PYY, or mRNA expressions of CCKa receptor and PYY3-36 receptor in the relevant tissues. The sham group, as a comparison group for AVNS, saw less effect in any of the indexes compared with the HFD group. AVNS had more effect on weight loss, reduction of perirenal WAT, and increase of NE, β3-adrenoceptor, and UCP1 than sham. Conclusions: AVNS was more effective in reducing body weight and causing visceral fat loss. Biochemical tests found more NE released in the serum and more β3-adrenoceptor and UCP1 expression in the BAT. All of these features suggested that energy expenditure might play an important role in obesity management by AVNS.

Original languageEnglish (US)
Pages (from-to)1416-1422
Number of pages7
JournalNutrition (Burbank, Los Angeles County, Calif.)
Volume31
Issue number11-12
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Vagus Nerve Stimulation
High Fat Diet
Brown Adipose Tissue
White Adipose Tissue
Adrenergic Receptors
Norepinephrine
Obesity
Gastric Emptying
Cholecystokinin
Body Weight
Energy Intake
Serum
Weights and Measures
Messenger RNA
Weight Loss
Genes
Cholecystokinin Receptors
Intra-Abdominal Fat
Ileum
Energy Metabolism

Keywords

  • Auricular vagus nerve stimulation
  • Energy expenditure
  • Food intake
  • Metabolism
  • Obesity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Han, L., Jian-Bin, Z., Chen, X., Qing-Qing, T., Wei-Xing, S., Jing-Zhu, Z., ... Yin-Ping, W. (2015). Effects and mechanisms of auricular vagus nerve stimulation on high-fat-diet-induced obese rats. Nutrition (Burbank, Los Angeles County, Calif.), 31(11-12), 1416-1422. https://doi.org/10.1016/j.nut.2015.05.007

Effects and mechanisms of auricular vagus nerve stimulation on high-fat-diet-induced obese rats. / Han, Li; Jian-Bin, Zhang; Chen, Xu; Qing-Qing, Tang; Wei-Xing, Shen; Jing-Zhu, Zhou; Chen, Jiande; Yin-Ping, Wang.

In: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 31, No. 11-12, 01.11.2015, p. 1416-1422.

Research output: Contribution to journalArticle

Han, L, Jian-Bin, Z, Chen, X, Qing-Qing, T, Wei-Xing, S, Jing-Zhu, Z, Chen, J & Yin-Ping, W 2015, 'Effects and mechanisms of auricular vagus nerve stimulation on high-fat-diet-induced obese rats', Nutrition (Burbank, Los Angeles County, Calif.), vol. 31, no. 11-12, pp. 1416-1422. https://doi.org/10.1016/j.nut.2015.05.007
Han, Li ; Jian-Bin, Zhang ; Chen, Xu ; Qing-Qing, Tang ; Wei-Xing, Shen ; Jing-Zhu, Zhou ; Chen, Jiande ; Yin-Ping, Wang. / Effects and mechanisms of auricular vagus nerve stimulation on high-fat-diet-induced obese rats. In: Nutrition (Burbank, Los Angeles County, Calif.). 2015 ; Vol. 31, No. 11-12. pp. 1416-1422.
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abstract = "Objective: Obesity is a major public health problem. Regulating food intake and promoting metabolism of fat are two important options for treating obesity. Auricular vagus nerve stimulation (AVNS) is considered as an alternative approach to vagal nerve stimulation. The aim of this study was to investigate the effects of AVNS and its mechanisms on obesity in obese rats. Methods: Male Sprague-Dawley rats were fed either a high-fat diet (HFD) or a normal diet for 8 wk. Qualified HFD rats were randomly divided into three groups: the HFD group, the AVNS group, and the sham group for 6 wk treatment. Body weight and daily energy intake were recorded weekly. The rats were sacrificed for measurement of weight of bilateral perirenal, epididymal white adipose tissue (WAT), dorsal brown adipose tissue (BAT), and gastric emptying. Serum cholecystokinin (CCK), peptide YY3 to 36 (PYY3-36) and norepinephrine (NE) were assayed by enzyme-linked immunosorbent assay. Real-time quantitative polymerase chain reaction was used to assess the mRNA expressions of CCK subtype receptor a (CCKa) in the antrum, PYY3-36 receptor in the distal ileum, β3-adrenoceptor, and uncoupling protein gene 1 (UCP1) in the BAT. Results: Compared with HFD group, AVNS significantly reduced body weight and epididymal WAT and increased BAT weight, serum NE, mRNA expressions of β3-adrenoceptors, and UCP1 of the BAT, but had no effect on daily energy intake, perirenal WAT weight, gastric emptying, serum levels of CCK and PYY, or mRNA expressions of CCKa receptor and PYY3-36 receptor in the relevant tissues. The sham group, as a comparison group for AVNS, saw less effect in any of the indexes compared with the HFD group. AVNS had more effect on weight loss, reduction of perirenal WAT, and increase of NE, β3-adrenoceptor, and UCP1 than sham. Conclusions: AVNS was more effective in reducing body weight and causing visceral fat loss. Biochemical tests found more NE released in the serum and more β3-adrenoceptor and UCP1 expression in the BAT. All of these features suggested that energy expenditure might play an important role in obesity management by AVNS.",
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AU - Jing-Zhu, Zhou

AU - Chen, Jiande

AU - Yin-Ping, Wang

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N2 - Objective: Obesity is a major public health problem. Regulating food intake and promoting metabolism of fat are two important options for treating obesity. Auricular vagus nerve stimulation (AVNS) is considered as an alternative approach to vagal nerve stimulation. The aim of this study was to investigate the effects of AVNS and its mechanisms on obesity in obese rats. Methods: Male Sprague-Dawley rats were fed either a high-fat diet (HFD) or a normal diet for 8 wk. Qualified HFD rats were randomly divided into three groups: the HFD group, the AVNS group, and the sham group for 6 wk treatment. Body weight and daily energy intake were recorded weekly. The rats were sacrificed for measurement of weight of bilateral perirenal, epididymal white adipose tissue (WAT), dorsal brown adipose tissue (BAT), and gastric emptying. Serum cholecystokinin (CCK), peptide YY3 to 36 (PYY3-36) and norepinephrine (NE) were assayed by enzyme-linked immunosorbent assay. Real-time quantitative polymerase chain reaction was used to assess the mRNA expressions of CCK subtype receptor a (CCKa) in the antrum, PYY3-36 receptor in the distal ileum, β3-adrenoceptor, and uncoupling protein gene 1 (UCP1) in the BAT. Results: Compared with HFD group, AVNS significantly reduced body weight and epididymal WAT and increased BAT weight, serum NE, mRNA expressions of β3-adrenoceptors, and UCP1 of the BAT, but had no effect on daily energy intake, perirenal WAT weight, gastric emptying, serum levels of CCK and PYY, or mRNA expressions of CCKa receptor and PYY3-36 receptor in the relevant tissues. The sham group, as a comparison group for AVNS, saw less effect in any of the indexes compared with the HFD group. AVNS had more effect on weight loss, reduction of perirenal WAT, and increase of NE, β3-adrenoceptor, and UCP1 than sham. Conclusions: AVNS was more effective in reducing body weight and causing visceral fat loss. Biochemical tests found more NE released in the serum and more β3-adrenoceptor and UCP1 expression in the BAT. All of these features suggested that energy expenditure might play an important role in obesity management by AVNS.

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