Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin

John R. Ticehurst, Deborah Z. Aird, Lisa M. Dam, Anita P. Borek, John T. Hargrove, Karen C. Carroll

Research output: Contribution to journalArticle

Abstract

We evaluated a two-step algorithm for detecting toxigenic Clostridium difficile: an enzyme immunoassay for glutamate dehydrogenase antigen (Ag-EIA) and then, for antigen-positive specimens, a concurrent cell culture cytotoxicity neutralization assay (CCNA). Antigen-negative results were ≥99% predictive of CCNA negativity. Because the Ag-EIA reduced cell culture workload by ≈75 to 80% and two-step testing was complete in ≤3 days, we decided that this algorithm would be effective. Over 6 months, our laboratories' expenses were US$143,000 less than if CCNA alone had been performed on all 5,887 specimens.

Original languageEnglish (US)
Pages (from-to)1145-1149
Number of pages5
JournalJournal of clinical microbiology
Volume44
Issue number3
DOIs
StatePublished - Mar 2006

ASJC Scopus subject areas

  • Microbiology (medical)

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