Abstract
We evaluated a two-step algorithm for detecting toxigenic Clostridium difficile: an enzyme immunoassay for glutamate dehydrogenase antigen (Ag-EIA) and then, for antigen-positive specimens, a concurrent cell culture cytotoxicity neutralization assay (CCNA). Antigen-negative results were ≥99% predictive of CCNA negativity. Because the Ag-EIA reduced cell culture workload by ≈75 to 80% and two-step testing was complete in ≤3 days, we decided that this algorithm would be effective. Over 6 months, our laboratories' expenses were US$143,000 less than if CCNA alone had been performed on all 5,887 specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1145-1149 |
| Number of pages | 5 |
| Journal | Journal of clinical microbiology |
| Volume | 44 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2006 |
ASJC Scopus subject areas
- Microbiology (medical)
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Cite this
Ticehurst, J. R., Aird, D. Z., Dam, L. M., Borek, A. P., Hargrove, J. T., & Carroll, K. C. (2006). Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. Journal of clinical microbiology, 44(3), 1145-1149. https://doi.org/10.1128/JCM.44.3.1145-1149.2006