TY - JOUR
T1 - Effective delivery of immunosuppressive drug molecules by silica coated iron oxide nanoparticles
AU - Hwang, Jangsun
AU - Lee, Eunwon
AU - Kim, Jieun
AU - Seo, Youngmin
AU - Lee, Kwan Hong
AU - Hong, Jong Wook
AU - Gilad, Assaf A.
AU - Park, Hansoo
AU - Choi, Jonghoon
N1 - Publisher Copyright:
© 2016 Elsevier B.V..
PY - 2016/6/1
Y1 - 2016/6/1
N2 - Iron oxide nanoparticles have been used in a wide range of biomedical applications, including drug delivery, molecular imaging, and cellular imaging. Various surface modifications have been applied to the particles to stabilize their surface and to give them a moiety for anchoring tags and/or drug molecules. Conventional methods of delivering immunosuppressant drugs often require a high dose of drugs to ensure therapeutic effects, but this can lead to toxic side effects. In this study, we used silica-coated iron oxide nanoparticles (IOSs) for a drug delivery application in which the nanoparticles carry the minimum amount of drug required to be effective to the target cells. IOSs could be loaded with water-insoluble immunosuppressive drug molecules (MPA: mycophenolic acid) and be used as a contrast agent for MRI. We characterized the IOSs for their physicochemical properties and found their average hydrodynamic diameter and core size to be 40.5 nm and 5 nm, respectively. Following the introduction of MPA-loaded IOSs (IOS/M), we evaluated the secretion dynamics of cytokines from peripheral blood mononuclear cells stimulated with phytohemagglutinin (PHA). The results showed that IOS/M effectively inhibited the secretion of the cytokines interleukin-2 and tumor necrosis factor α, with a minimal concentration of MPA. In conclusion, IOS/M may have potential applications in both efficient drug delivery and MRI.
AB - Iron oxide nanoparticles have been used in a wide range of biomedical applications, including drug delivery, molecular imaging, and cellular imaging. Various surface modifications have been applied to the particles to stabilize their surface and to give them a moiety for anchoring tags and/or drug molecules. Conventional methods of delivering immunosuppressant drugs often require a high dose of drugs to ensure therapeutic effects, but this can lead to toxic side effects. In this study, we used silica-coated iron oxide nanoparticles (IOSs) for a drug delivery application in which the nanoparticles carry the minimum amount of drug required to be effective to the target cells. IOSs could be loaded with water-insoluble immunosuppressive drug molecules (MPA: mycophenolic acid) and be used as a contrast agent for MRI. We characterized the IOSs for their physicochemical properties and found their average hydrodynamic diameter and core size to be 40.5 nm and 5 nm, respectively. Following the introduction of MPA-loaded IOSs (IOS/M), we evaluated the secretion dynamics of cytokines from peripheral blood mononuclear cells stimulated with phytohemagglutinin (PHA). The results showed that IOS/M effectively inhibited the secretion of the cytokines interleukin-2 and tumor necrosis factor α, with a minimal concentration of MPA. In conclusion, IOS/M may have potential applications in both efficient drug delivery and MRI.
KW - Drug delivery
KW - Immunosuppressive drug
KW - Iron oxide nanoparticles
KW - Magnetic resonance imaging
KW - Mycophenolic acid
UR - http://www.scopus.com/inward/record.url?scp=84960123324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84960123324&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2016.01.040
DO - 10.1016/j.colsurfb.2016.01.040
M3 - Article
C2 - 26966999
AN - SCOPUS:84960123324
SN - 0927-7765
VL - 142
SP - 290
EP - 296
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -