Three distinct mucopolysaccharide-peptide complexes have been isolated from lung tissue and identified by the following procedures as hyaluronic acid, a sulfated hyaluronic acid, and heparan sulfate: elution pattern from DEAE-Sephadex columns and paper electrophoresis; analysis of chemical composition; and susceptibility or resistance to hyaluronidase or heparinase digestion. The incorporation of 35SO2-4 and glucosamine-1-14C into these mucopolysaccharides and into cartilage chondroitin sulfate of vitamin A-normal and vitamin A-deficient pair-fed rats was studied. The amount of each compound was higher in the vitamin A-deficient animals. The distribution of label in the unsulfated and less sulfated mucopolysaccharides showed higher values in the vitamin A-deficient animals. The lung heparan sulfate and inter-costal cartilage chondroitin sulfate, on the other hand, had a higher incorporation of labeled sulfate in vitamin A-normal compared with vitamin A-deficient animals.
ASJC Scopus subject areas
- Molecular Biology