Effect of uremia on rates of ethanol disappearance from the blood and on the activities of the ethanol-oxidizing enzymes

Esteban Mezey, Robert E. Vestal, James John Potter, John W. Rowe

Research output: Contribution to journalArticle

Abstract

The effect of uremia on ethanol metabolism was investigated. Uremia was induced in male Sprague-Dawley rats by removal of approximately 85 per cent of the renal mass. Control animals had a sham operation. The mean activity of alcohol dehydrogenase was markedly increased in the uremic rats to 2.12 ± 0.13 (S.E.M.) μmoles per milligram of protein per hour as compared with a control value of 1.39 ± 0.13 μmoles per milligram of protein per hour (p <0.001). There were no changes in the activity of the microsomal ethanol oxidizing system, in catalase activity present in the microsomes, or in the rates of ethanol disappearance from the blood. Uremia resulted in decreases in microsomal cytochrome P-450, but no changes in cytochrome b5, NADPH-cytochrome c reductase, or in the activities of aniline hydroxylase and aminopyrine demethylase. The increase in alcohol dehydrogenase activity could not be reproduced by incubation of liver from a normal rat with uremic rat plasma, uremic human serum, or urea. Also, the increase in the enzyme activity was not associated with changes in leucocyte ascorbic acid levels. The cause and physiologic significance of the increase in alcohol dehydrogenase activity in uremia remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)931-937
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Volume86
Issue number6
StatePublished - 1975

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Uremia
Rats
Alcohol Dehydrogenase
Blood
Ethanol
Enzymes
Aniline Hydroxylase
Plasma (human)
Aminopyrine
Cytochromes b5
NADPH-Ferrihemoprotein Reductase
Enzyme activity
Microsomes
Metabolism
Liver
Catalase
Cytochrome P-450 Enzyme System
Ascorbic Acid
Sprague Dawley Rats
Urea

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

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title = "Effect of uremia on rates of ethanol disappearance from the blood and on the activities of the ethanol-oxidizing enzymes",
abstract = "The effect of uremia on ethanol metabolism was investigated. Uremia was induced in male Sprague-Dawley rats by removal of approximately 85 per cent of the renal mass. Control animals had a sham operation. The mean activity of alcohol dehydrogenase was markedly increased in the uremic rats to 2.12 ± 0.13 (S.E.M.) μmoles per milligram of protein per hour as compared with a control value of 1.39 ± 0.13 μmoles per milligram of protein per hour (p <0.001). There were no changes in the activity of the microsomal ethanol oxidizing system, in catalase activity present in the microsomes, or in the rates of ethanol disappearance from the blood. Uremia resulted in decreases in microsomal cytochrome P-450, but no changes in cytochrome b5, NADPH-cytochrome c reductase, or in the activities of aniline hydroxylase and aminopyrine demethylase. The increase in alcohol dehydrogenase activity could not be reproduced by incubation of liver from a normal rat with uremic rat plasma, uremic human serum, or urea. Also, the increase in the enzyme activity was not associated with changes in leucocyte ascorbic acid levels. The cause and physiologic significance of the increase in alcohol dehydrogenase activity in uremia remain to be elucidated.",
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AU - Rowe, John W.

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N2 - The effect of uremia on ethanol metabolism was investigated. Uremia was induced in male Sprague-Dawley rats by removal of approximately 85 per cent of the renal mass. Control animals had a sham operation. The mean activity of alcohol dehydrogenase was markedly increased in the uremic rats to 2.12 ± 0.13 (S.E.M.) μmoles per milligram of protein per hour as compared with a control value of 1.39 ± 0.13 μmoles per milligram of protein per hour (p <0.001). There were no changes in the activity of the microsomal ethanol oxidizing system, in catalase activity present in the microsomes, or in the rates of ethanol disappearance from the blood. Uremia resulted in decreases in microsomal cytochrome P-450, but no changes in cytochrome b5, NADPH-cytochrome c reductase, or in the activities of aniline hydroxylase and aminopyrine demethylase. The increase in alcohol dehydrogenase activity could not be reproduced by incubation of liver from a normal rat with uremic rat plasma, uremic human serum, or urea. Also, the increase in the enzyme activity was not associated with changes in leucocyte ascorbic acid levels. The cause and physiologic significance of the increase in alcohol dehydrogenase activity in uremia remain to be elucidated.

AB - The effect of uremia on ethanol metabolism was investigated. Uremia was induced in male Sprague-Dawley rats by removal of approximately 85 per cent of the renal mass. Control animals had a sham operation. The mean activity of alcohol dehydrogenase was markedly increased in the uremic rats to 2.12 ± 0.13 (S.E.M.) μmoles per milligram of protein per hour as compared with a control value of 1.39 ± 0.13 μmoles per milligram of protein per hour (p <0.001). There were no changes in the activity of the microsomal ethanol oxidizing system, in catalase activity present in the microsomes, or in the rates of ethanol disappearance from the blood. Uremia resulted in decreases in microsomal cytochrome P-450, but no changes in cytochrome b5, NADPH-cytochrome c reductase, or in the activities of aniline hydroxylase and aminopyrine demethylase. The increase in alcohol dehydrogenase activity could not be reproduced by incubation of liver from a normal rat with uremic rat plasma, uremic human serum, or urea. Also, the increase in the enzyme activity was not associated with changes in leucocyte ascorbic acid levels. The cause and physiologic significance of the increase in alcohol dehydrogenase activity in uremia remain to be elucidated.

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