Effect of treatment on serum antibody to Hymenolepsis nana detected by enzyme-linked immunosorbent assay

R. M. Castillo, P. Grados, C. Carcamo, E. Miranda, T. Montenegro, A. Guevara, Robert H Gilman

Research output: Contribution to journalArticle

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to measure serum immunoglobulin G antibodies in 65 patients infected with Hymenolepis nana and 30 noninfected patients. Antibody was detected in 51 of 65 (sensitivity, 79%) and 5 of 30 H. nana-negative patients (specificity, 83%). Nine patients infected with H. nana were treated with praziquantel (20 to 25 mg/kg of body weight). Antibody disappeared from the sera at 90 days in six patients, five of whom had eliminated H. nana. Antibody persisted in three patients in whom H. nana infection did not clear after treatment. The H. nana ELISA had a high rate of cross-reactions with sera from patients with cysticercosis (8 of 29 [28%]) and hydatidosis (8 of 23 [35%]). The ELISA for H. nana may be useful for defining the epidemiology of H. nana infections, especially in areas free from cysticercosis and hydatidosis.

Original languageEnglish (US)
Pages (from-to)413-414
Number of pages2
JournalJournal of Clinical Microbiology
Volume29
Issue number2
StatePublished - 1991
Externally publishedYes

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Hymenolepis nana
Enzyme-Linked Immunosorbent Assay
Antibodies
Serum
Hymenolepiasis
Cysticercosis
Echinococcosis
Therapeutics
Praziquantel
Cross Reactions
Epidemiology
Immunoglobulin G
Body Weight

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Castillo, R. M., Grados, P., Carcamo, C., Miranda, E., Montenegro, T., Guevara, A., & Gilman, R. H. (1991). Effect of treatment on serum antibody to Hymenolepsis nana detected by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, 29(2), 413-414.

Effect of treatment on serum antibody to Hymenolepsis nana detected by enzyme-linked immunosorbent assay. / Castillo, R. M.; Grados, P.; Carcamo, C.; Miranda, E.; Montenegro, T.; Guevara, A.; Gilman, Robert H.

In: Journal of Clinical Microbiology, Vol. 29, No. 2, 1991, p. 413-414.

Research output: Contribution to journalArticle

Castillo, RM, Grados, P, Carcamo, C, Miranda, E, Montenegro, T, Guevara, A & Gilman, RH 1991, 'Effect of treatment on serum antibody to Hymenolepsis nana detected by enzyme-linked immunosorbent assay', Journal of Clinical Microbiology, vol. 29, no. 2, pp. 413-414.
Castillo RM, Grados P, Carcamo C, Miranda E, Montenegro T, Guevara A et al. Effect of treatment on serum antibody to Hymenolepsis nana detected by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology. 1991;29(2):413-414.
Castillo, R. M. ; Grados, P. ; Carcamo, C. ; Miranda, E. ; Montenegro, T. ; Guevara, A. ; Gilman, Robert H. / Effect of treatment on serum antibody to Hymenolepsis nana detected by enzyme-linked immunosorbent assay. In: Journal of Clinical Microbiology. 1991 ; Vol. 29, No. 2. pp. 413-414.
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