Effect of the exposure of intrafollicular oocytes to clomiphene citrate on pregnancy outcome in the rabbit

Y. Yoshimura; Y. Hosoi; S. J. Atlas; A. M. Dharmarajan; T. Adachi; E. E. Wallach

doi:10.1016/s0015-0282(16)60023-7

Effect of the exposure of intrafollicular oocytes to clomiphene citrate on pregnancy outcome in the rabbit

Y. Yoshimura, Y. Hosoi, S. J. Atlas, A. M. Dharmarajan, T. Adachi, E. E. Wallach

School of Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This study was designed to determine if exposure of rabbit intrafollicular oocytes to clomiphene citrate (CC) affects pregnancy outcome after in vitro ovulation, in vitro fertilization (IVF), and embryo transfer (ET). Ovaries were perfused in the presence or absence of CC (10^-5 M) and estradiol (E₂, 100 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of all ovaries. In vitro ovulated ova were retrieved, inseminated, and transferred to host rabbits. Neither CC nor CC + E₂ significantly affected hCG-induced ovulation or fertilization. CC significantly reduced (P < 0.025) percentage of ovulated ova resulting in offspring. Addition of E₂ significantly (P < 0.05) reversed the reduction in offspring associated with CC alone. These results may be due to the antiestrogenic effects of CC on the intrafollicular oocyte, which compromises postfertilization development.

Original language	English (US)
Pages (from-to)	153-158
Number of pages	6
Journal	Fertility and sterility
Volume	50
Issue number	1
DOIs	https://doi.org/10.1016/s0015-0282(16)60023-7
State	Published - 1988

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/s0015-0282(16)60023-7

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title = "Effect of the exposure of intrafollicular oocytes to clomiphene citrate on pregnancy outcome in the rabbit",

abstract = "This study was designed to determine if exposure of rabbit intrafollicular oocytes to clomiphene citrate (CC) affects pregnancy outcome after in vitro ovulation, in vitro fertilization (IVF), and embryo transfer (ET). Ovaries were perfused in the presence or absence of CC (10-5 M) and estradiol (E2, 100 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of all ovaries. In vitro ovulated ova were retrieved, inseminated, and transferred to host rabbits. Neither CC nor CC + E2 significantly affected hCG-induced ovulation or fertilization. CC significantly reduced (P < 0.025) percentage of ovulated ova resulting in offspring. Addition of E2 significantly (P < 0.05) reversed the reduction in offspring associated with CC alone. These results may be due to the antiestrogenic effects of CC on the intrafollicular oocyte, which compromises postfertilization development.",

author = "Y. Yoshimura and Y. Hosoi and Atlas, {S. J.} and Dharmarajan, {A. M.} and T. Adachi and Wallach, {E. E.}",

note = "Funding Information: Received December 10, 1987; revised and accepted March 16, 1988. *Supported by the National Institutes of Health grant HD 19430 and the Connelly Foundation. t Presented in part at the 43rd Annual Meeting of the American Fertility Society, Reno, Nevada, September 28 to 30, 1987. :j: Reprint requests: Edward E. Wallach, M.D., Department of Gynecology and Obstetrics, The Johns Hopkins Medical Institutions, 264 Houck Building, 600 N. Wolfe Street, Baltimore, Maryland 21205.",

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doi = "10.1016/s0015-0282(16)60023-7",

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AU - Yoshimura, Y.

AU - Hosoi, Y.

AU - Atlas, S. J.

AU - Dharmarajan, A. M.

AU - Adachi, T.

AU - Wallach, E. E.

N1 - Funding Information: Received December 10, 1987; revised and accepted March 16, 1988. *Supported by the National Institutes of Health grant HD 19430 and the Connelly Foundation. t Presented in part at the 43rd Annual Meeting of the American Fertility Society, Reno, Nevada, September 28 to 30, 1987. :j: Reprint requests: Edward E. Wallach, M.D., Department of Gynecology and Obstetrics, The Johns Hopkins Medical Institutions, 264 Houck Building, 600 N. Wolfe Street, Baltimore, Maryland 21205.

PY - 1988

Y1 - 1988

N2 - This study was designed to determine if exposure of rabbit intrafollicular oocytes to clomiphene citrate (CC) affects pregnancy outcome after in vitro ovulation, in vitro fertilization (IVF), and embryo transfer (ET). Ovaries were perfused in the presence or absence of CC (10-5 M) and estradiol (E2, 100 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of all ovaries. In vitro ovulated ova were retrieved, inseminated, and transferred to host rabbits. Neither CC nor CC + E2 significantly affected hCG-induced ovulation or fertilization. CC significantly reduced (P < 0.025) percentage of ovulated ova resulting in offspring. Addition of E2 significantly (P < 0.05) reversed the reduction in offspring associated with CC alone. These results may be due to the antiestrogenic effects of CC on the intrafollicular oocyte, which compromises postfertilization development.

AB - This study was designed to determine if exposure of rabbit intrafollicular oocytes to clomiphene citrate (CC) affects pregnancy outcome after in vitro ovulation, in vitro fertilization (IVF), and embryo transfer (ET). Ovaries were perfused in the presence or absence of CC (10-5 M) and estradiol (E2, 100 ng/ml). Human chorionic gonadotropin (hCG, 50 IU) was added to the perfusate of all ovaries. In vitro ovulated ova were retrieved, inseminated, and transferred to host rabbits. Neither CC nor CC + E2 significantly affected hCG-induced ovulation or fertilization. CC significantly reduced (P < 0.025) percentage of ovulated ova resulting in offspring. Addition of E2 significantly (P < 0.05) reversed the reduction in offspring associated with CC alone. These results may be due to the antiestrogenic effects of CC on the intrafollicular oocyte, which compromises postfertilization development.

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Effect of the exposure of intrafollicular oocytes to clomiphene citrate on pregnancy outcome in the rabbit

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