Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp.

Cristina K. Moreira, Flávia G. Rodrigues, Anil Ghosh, Fernando de P Varotti, Antonio Miranda, Sirlei Daffre, Marcelo Jacobs-Lorena, Luciano A. Moreira

Research output: Contribution to journalArticle

Abstract

While seeking strategies for interfering with Plasmodium development in vertebrate/invertebrate hosts, we tested the activity of gomesin, an antimicrobial peptide isolated from the hemocytes of the spider Acanthoscurria gomesiana. Gomesin was tested against asexual, sexual and pre-sporogonic forms of Plasmodium falciparum and Plasmodium berghei parasites. The peptide inhibited the in vitro growth of intraerythrocytic forms of P. falciparum.When gomesin was added to in vitro culture of P. berghei mature gametocytes, it significantly inhibited the exflagellation of male gametes and the formation of ookinetes. In vivo, the peptide reduced the number of oocysts of both Plasmodium species in Anopheles stephensi mosquitoes, and did not appear to affect the mosquitoes. These properties make gomesin an excellent candidate as a transmission blocking agent for the genetic engineering of mosquitoes.

Original languageEnglish (US)
Pages (from-to)346-353
Number of pages8
JournalExperimental Parasitology
Volume116
Issue number4
DOIs
StatePublished - Aug 2007

Fingerprint

Plasmodium
Culicidae
Plasmodium berghei
Peptides
Plasmodium falciparum
Hemocytes
Spiders
Anopheles
Genetic Engineering
Oocysts
Invertebrates
Germ Cells
Vertebrates
Parasites
gomesin
Growth
In Vitro Techniques

Keywords

  • American Type Culture Collection
  • An. stephensi
  • Anopheles stephensi
  • Antimicrobial peptide
  • ATCC
  • Exflagellation
  • FIOCRUZ
  • Fundação Oswaldo Cruz
  • GFP
  • Gomesin
  • green fluorescent protein
  • Malaria
  • Malaria Research and Reference Reagent Resource Center
  • Mosquito
  • MR4
  • Oocyst
  • Ookinete
  • P. berghei
  • P. falciparum
  • Plasmodium berghei
  • Plasmodium falciparum
  • Roswell Park Memorial Institute medium
  • RPMI-1640
  • t-Boc
  • tert-butyloxycarbonyl

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Moreira, C. K., Rodrigues, F. G., Ghosh, A., Varotti, F. D. P., Miranda, A., Daffre, S., ... Moreira, L. A. (2007). Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp. Experimental Parasitology, 116(4), 346-353. https://doi.org/10.1016/j.exppara.2007.01.022

Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp. / Moreira, Cristina K.; Rodrigues, Flávia G.; Ghosh, Anil; Varotti, Fernando de P; Miranda, Antonio; Daffre, Sirlei; Jacobs-Lorena, Marcelo; Moreira, Luciano A.

In: Experimental Parasitology, Vol. 116, No. 4, 08.2007, p. 346-353.

Research output: Contribution to journalArticle

Moreira, CK, Rodrigues, FG, Ghosh, A, Varotti, FDP, Miranda, A, Daffre, S, Jacobs-Lorena, M & Moreira, LA 2007, 'Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp.', Experimental Parasitology, vol. 116, no. 4, pp. 346-353. https://doi.org/10.1016/j.exppara.2007.01.022
Moreira, Cristina K. ; Rodrigues, Flávia G. ; Ghosh, Anil ; Varotti, Fernando de P ; Miranda, Antonio ; Daffre, Sirlei ; Jacobs-Lorena, Marcelo ; Moreira, Luciano A. / Effect of the antimicrobial peptide gomesin against different life stages of Plasmodium spp. In: Experimental Parasitology. 2007 ; Vol. 116, No. 4. pp. 346-353.
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