Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe 3+) by ascorbate

Jianjing Tong, Joseph R. Zweier, Rachael L. Huskey, Raed S. Ismail, Craig Hemann, Jay L. Zweier, Xiaoping Liu

Research output: Contribution to journalArticle

Abstract

Cytoglobin (Cygb) plays a role in regulating vasodilation in response to changes in local oxygen concentration by altering the rate of nitric oxide (NO) metabolism. Because the reduction of Cygb(Fe3+) by a reductant is the control step for Cygb-mediated NO metabolism, we examined the effects of temperature, pH, and heme ligands on the Cygb(Fe3+) reduction by ascorbate (Asc) under anaerobic conditions. The standard enthalpy of Cygb(Fe3+) reduction by Asc was determined to be 42.4 ± 3.1 kJ/mol. The rate of Cygb(Fe3+) reduction increased ∼6% per °C when temperature varied from 35°C to 40°C. The yield and the rate of Cygb(Fe3+) reduction significantly increases with pH (2-3 times per pH unit), paralleling the formation of the Asc ion (A2-) and the increased stability of reduced state of heme iron at high pH values. Heme ligand cyanide (CN-) decreased the yield and the rate of Cygb(Fe 3+) reduction, but ligands CO and NO allowed the process of Cygb(Fe3+) reduction to continue to completion. Critical information is provided for modeling and prediction of the process of Cygb-mediated NO metabolism in vessels in a range of temperature and pH values.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume554
DOIs
StatePublished - Jul 15 2014
Externally publishedYes

Fingerprint

Heme
Ligands
Temperature
Nitric Oxide
Metabolism
iron(III)-ascorbic acid complex
cytoglobin
Reducing Agents
Cyanides
Carbon Monoxide
varespladib methyl
Vasodilation
Enthalpy
Iron
Ions
Oxygen

Keywords

  • Ascorbate
  • Cytoglobin
  • Heme ligand
  • pH
  • Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Tong, J., Zweier, J. R., Huskey, R. L., Ismail, R. S., Hemann, C., Zweier, J. L., & Liu, X. (2014). Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe 3+) by ascorbate. Archives of Biochemistry and Biophysics, 554, 1-5. https://doi.org/10.1016/j.abb.2014.04.011

Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe 3+) by ascorbate. / Tong, Jianjing; Zweier, Joseph R.; Huskey, Rachael L.; Ismail, Raed S.; Hemann, Craig; Zweier, Jay L.; Liu, Xiaoping.

In: Archives of Biochemistry and Biophysics, Vol. 554, 15.07.2014, p. 1-5.

Research output: Contribution to journalArticle

Tong, Jianjing ; Zweier, Joseph R. ; Huskey, Rachael L. ; Ismail, Raed S. ; Hemann, Craig ; Zweier, Jay L. ; Liu, Xiaoping. / Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe 3+) by ascorbate. In: Archives of Biochemistry and Biophysics. 2014 ; Vol. 554. pp. 1-5.
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