Effect of surgical procedures on prostate tumor gene expression profiles

Jie Li, Zhi Hong Zhang, Chang Jun Yin, Christian Pavlovich, Jun Luo, Robert Getzenberg, Wei Zhang

Research output: Contribution to journalArticle

Abstract

Current surgical treatment of prostate cancer is typically accomplished by either open radical prostatectomy (ORP) or robotic-assisted laparoscopic radical prostatectomy (RALRP). Intra-operative procedural differences between the two surgical approaches may alter the molecular composition of resected surgical specimens, which are indispensable for molecular analysis and biomarker evaluation. The objective of this study is to investigate the effect of different surgical procedures on RNA quality and genome-wide expression signature. RNA integrity number (RIN) values were compared between total RNA samples extracted from consecutive LRP (n11) and ORP (n24) prostate specimens. Expression profiling was performed using the Agilent human whole-genome expression microarrays. Expression differences by surgical type were analyzed by Volcano plot analysis and gene ontology analysis. Quantitative reverse transcription (RT)-PCR was used for expression validation in an independent set of LRP (n8) and ORP (n8) samples. The LRP procedure did not compromise RNA integrity. Differential gene expression by surgery types was limited to a small subset of genes, the number of which was smaller than that expected by chance. Unexpectedly, this small subset of differentially expressed genes was enriched for those encoding transcription factors, oxygen transporters and other previously reported surgery-induced stress-response genes, and demonstrated unidirectional reduction in LRP specimens in comparison to ORP specimens. The effect of the LRP procedure on RNA quality and genome-wide transcript levels is negligible, supporting the suitability of LRP surgical specimens for routine molecular analysis. Blunted in vivo stress response in LRP specimens, likely mediated by CO 2 insufflation but not by longer ischemia time, is manifested in the reduced expression of stress-response genes in these specimens.

Original languageEnglish (US)
Pages (from-to)708-714
Number of pages7
JournalAsian Journal of Andrology
Volume14
Issue number5
DOIs
StatePublished - Sep 2012

Fingerprint

Prostatectomy
Transcriptome
Prostate
RNA
Neoplasms
Genes
Genome
Insufflation
Gene Ontology
Robotics
Human Genome
Carbon Monoxide
Reverse Transcription
Prostatic Neoplasms
Transcription Factors
Ischemia
Biomarkers
Oxygen
Gene Expression
Polymerase Chain Reaction

Keywords

  • CO2 insufflation
  • expression microarray
  • laparoscopic radical prostatectomy
  • open radical prostatectomy
  • prostate cancer
  • stress response

ASJC Scopus subject areas

  • Urology

Cite this

Effect of surgical procedures on prostate tumor gene expression profiles. / Li, Jie; Zhang, Zhi Hong; Yin, Chang Jun; Pavlovich, Christian; Luo, Jun; Getzenberg, Robert; Zhang, Wei.

In: Asian Journal of Andrology, Vol. 14, No. 5, 09.2012, p. 708-714.

Research output: Contribution to journalArticle

Li, Jie ; Zhang, Zhi Hong ; Yin, Chang Jun ; Pavlovich, Christian ; Luo, Jun ; Getzenberg, Robert ; Zhang, Wei. / Effect of surgical procedures on prostate tumor gene expression profiles. In: Asian Journal of Andrology. 2012 ; Vol. 14, No. 5. pp. 708-714.
@article{d043d1808f3a47bc80d7bd9d2f9d68a2,
title = "Effect of surgical procedures on prostate tumor gene expression profiles",
abstract = "Current surgical treatment of prostate cancer is typically accomplished by either open radical prostatectomy (ORP) or robotic-assisted laparoscopic radical prostatectomy (RALRP). Intra-operative procedural differences between the two surgical approaches may alter the molecular composition of resected surgical specimens, which are indispensable for molecular analysis and biomarker evaluation. The objective of this study is to investigate the effect of different surgical procedures on RNA quality and genome-wide expression signature. RNA integrity number (RIN) values were compared between total RNA samples extracted from consecutive LRP (n11) and ORP (n24) prostate specimens. Expression profiling was performed using the Agilent human whole-genome expression microarrays. Expression differences by surgical type were analyzed by Volcano plot analysis and gene ontology analysis. Quantitative reverse transcription (RT)-PCR was used for expression validation in an independent set of LRP (n8) and ORP (n8) samples. The LRP procedure did not compromise RNA integrity. Differential gene expression by surgery types was limited to a small subset of genes, the number of which was smaller than that expected by chance. Unexpectedly, this small subset of differentially expressed genes was enriched for those encoding transcription factors, oxygen transporters and other previously reported surgery-induced stress-response genes, and demonstrated unidirectional reduction in LRP specimens in comparison to ORP specimens. The effect of the LRP procedure on RNA quality and genome-wide transcript levels is negligible, supporting the suitability of LRP surgical specimens for routine molecular analysis. Blunted in vivo stress response in LRP specimens, likely mediated by CO 2 insufflation but not by longer ischemia time, is manifested in the reduced expression of stress-response genes in these specimens.",
keywords = "CO2 insufflation, expression microarray, laparoscopic radical prostatectomy, open radical prostatectomy, prostate cancer, stress response",
author = "Jie Li and Zhang, {Zhi Hong} and Yin, {Chang Jun} and Christian Pavlovich and Jun Luo and Robert Getzenberg and Wei Zhang",
year = "2012",
month = "9",
doi = "10.1038/aja.2012.54",
language = "English (US)",
volume = "14",
pages = "708--714",
journal = "Asian Journal of Andrology",
issn = "1008-682X",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Effect of surgical procedures on prostate tumor gene expression profiles

AU - Li, Jie

AU - Zhang, Zhi Hong

AU - Yin, Chang Jun

AU - Pavlovich, Christian

AU - Luo, Jun

AU - Getzenberg, Robert

AU - Zhang, Wei

PY - 2012/9

Y1 - 2012/9

N2 - Current surgical treatment of prostate cancer is typically accomplished by either open radical prostatectomy (ORP) or robotic-assisted laparoscopic radical prostatectomy (RALRP). Intra-operative procedural differences between the two surgical approaches may alter the molecular composition of resected surgical specimens, which are indispensable for molecular analysis and biomarker evaluation. The objective of this study is to investigate the effect of different surgical procedures on RNA quality and genome-wide expression signature. RNA integrity number (RIN) values were compared between total RNA samples extracted from consecutive LRP (n11) and ORP (n24) prostate specimens. Expression profiling was performed using the Agilent human whole-genome expression microarrays. Expression differences by surgical type were analyzed by Volcano plot analysis and gene ontology analysis. Quantitative reverse transcription (RT)-PCR was used for expression validation in an independent set of LRP (n8) and ORP (n8) samples. The LRP procedure did not compromise RNA integrity. Differential gene expression by surgery types was limited to a small subset of genes, the number of which was smaller than that expected by chance. Unexpectedly, this small subset of differentially expressed genes was enriched for those encoding transcription factors, oxygen transporters and other previously reported surgery-induced stress-response genes, and demonstrated unidirectional reduction in LRP specimens in comparison to ORP specimens. The effect of the LRP procedure on RNA quality and genome-wide transcript levels is negligible, supporting the suitability of LRP surgical specimens for routine molecular analysis. Blunted in vivo stress response in LRP specimens, likely mediated by CO 2 insufflation but not by longer ischemia time, is manifested in the reduced expression of stress-response genes in these specimens.

AB - Current surgical treatment of prostate cancer is typically accomplished by either open radical prostatectomy (ORP) or robotic-assisted laparoscopic radical prostatectomy (RALRP). Intra-operative procedural differences between the two surgical approaches may alter the molecular composition of resected surgical specimens, which are indispensable for molecular analysis and biomarker evaluation. The objective of this study is to investigate the effect of different surgical procedures on RNA quality and genome-wide expression signature. RNA integrity number (RIN) values were compared between total RNA samples extracted from consecutive LRP (n11) and ORP (n24) prostate specimens. Expression profiling was performed using the Agilent human whole-genome expression microarrays. Expression differences by surgical type were analyzed by Volcano plot analysis and gene ontology analysis. Quantitative reverse transcription (RT)-PCR was used for expression validation in an independent set of LRP (n8) and ORP (n8) samples. The LRP procedure did not compromise RNA integrity. Differential gene expression by surgery types was limited to a small subset of genes, the number of which was smaller than that expected by chance. Unexpectedly, this small subset of differentially expressed genes was enriched for those encoding transcription factors, oxygen transporters and other previously reported surgery-induced stress-response genes, and demonstrated unidirectional reduction in LRP specimens in comparison to ORP specimens. The effect of the LRP procedure on RNA quality and genome-wide transcript levels is negligible, supporting the suitability of LRP surgical specimens for routine molecular analysis. Blunted in vivo stress response in LRP specimens, likely mediated by CO 2 insufflation but not by longer ischemia time, is manifested in the reduced expression of stress-response genes in these specimens.

KW - CO2 insufflation

KW - expression microarray

KW - laparoscopic radical prostatectomy

KW - open radical prostatectomy

KW - prostate cancer

KW - stress response

UR - http://www.scopus.com/inward/record.url?scp=84866256361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866256361&partnerID=8YFLogxK

U2 - 10.1038/aja.2012.54

DO - 10.1038/aja.2012.54

M3 - Article

C2 - 22864281

AN - SCOPUS:84866256361

VL - 14

SP - 708

EP - 714

JO - Asian Journal of Andrology

JF - Asian Journal of Andrology

SN - 1008-682X

IS - 5

ER -