Effect of STAT5b on rat liver alcohol dehydrogenase

J. J. Potter; E. Mezey

doi:10.1006/abbi.2001.2393

Effect of STAT5b on rat liver alcohol dehydrogenase

J. J. Potter, E. Mezey

School of Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Growth hormone (GH) enhances rat liver alcohol dehydrogenase (ADH) due to an increase in enzyme synthesis, which is mediated at the level of transcription. Previous studies have shown that the effect of GH in enhancing activation of the ADH promoter is mediated by C/EBPβ binding to region -22 to -11 relative to the start of transcription. In this study, STAT5b and C/EBPβ were found to bind to adjacent nucleotide sequences on a region between -226 and -194. Expression vectors for both STAT5b and C/EBPβ independently activated the promoter. Furthermore, the expression vector for the GH receptor also activated the ADH promoter, and this effect was abrogated by mutations of the adjacent STAT5b and C/EBPβ binding sites. These observations indicate that the enhancing effect of GH is mediated by both STAT5b and C/EBPβ.

Original language	English (US)
Pages (from-to)	41-48
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	391
Issue number	1
DOIs	https://doi.org/10.1006/abbi.2001.2393
State	Published - Jul 1 2001

Keywords

Alcohol dehydrogenase
C/EBPβ
Growth hormone
STAT5b

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1006/abbi.2001.2393

Cite this

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title = "Effect of STAT5b on rat liver alcohol dehydrogenase",

abstract = "Growth hormone (GH) enhances rat liver alcohol dehydrogenase (ADH) due to an increase in enzyme synthesis, which is mediated at the level of transcription. Previous studies have shown that the effect of GH in enhancing activation of the ADH promoter is mediated by C/EBPβ binding to region -22 to -11 relative to the start of transcription. In this study, STAT5b and C/EBPβ were found to bind to adjacent nucleotide sequences on a region between -226 and -194. Expression vectors for both STAT5b and C/EBPβ independently activated the promoter. Furthermore, the expression vector for the GH receptor also activated the ADH promoter, and this effect was abrogated by mutations of the adjacent STAT5b and C/EBPβ binding sites. These observations indicate that the enhancing effect of GH is mediated by both STAT5b and C/EBPβ.",

keywords = "Alcohol dehydrogenase, C/EBPβ, Growth hormone, STAT5b",

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AU - Mezey, E.

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N2 - Growth hormone (GH) enhances rat liver alcohol dehydrogenase (ADH) due to an increase in enzyme synthesis, which is mediated at the level of transcription. Previous studies have shown that the effect of GH in enhancing activation of the ADH promoter is mediated by C/EBPβ binding to region -22 to -11 relative to the start of transcription. In this study, STAT5b and C/EBPβ were found to bind to adjacent nucleotide sequences on a region between -226 and -194. Expression vectors for both STAT5b and C/EBPβ independently activated the promoter. Furthermore, the expression vector for the GH receptor also activated the ADH promoter, and this effect was abrogated by mutations of the adjacent STAT5b and C/EBPβ binding sites. These observations indicate that the enhancing effect of GH is mediated by both STAT5b and C/EBPβ.

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Effect of STAT5b on rat liver alcohol dehydrogenase

Abstract

Keywords

ASJC Scopus subject areas

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