Abstract
Hormonal induction of growth-arrested 3T3-L1 preadipocytes in medium containing fetal bovine serum (FBS) triggers a signaling cascade that culminates in adipogenesis. The transcription factor, C/EBPβ, is expressed early in this differentiation program, but lacks DNA-binding activity until much later as the preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Dominant-negative CHOP-10 is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPβ by heterodimerization with its leucine zipper. After a lag period, CHOP-10 undergoes down-regulation releasing C/EBPβ from inhibitory constraint allowing transactivation of the C/EBPα and PPARγ genes, transcription factors required for terminal differentiation. We verify that following induction of differentiation in FBS-containing medium, CHOP-10 undergoes down-regulation and differentiation occurs normally. However, when differentiation is induced in calf serum-containing medium, CHOP-10 is not down-regulated resulting in delayed and incomplete differentiation. Under these conditions the expression of C/EBPα and PPARγ and the accumulation of cytoplasmic triglyceride are attenuated. It appears that a factor(s) present in FBS is required to affect the down-regulation of CHOP-10 necessary for successful terminal differentiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1185-1188 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 338 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 16 2005 |
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Keywords
- Adipocyte differentiation
- C/EBPα
- C/EBPβ
- PPARγ
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Effect of serum on the down-regulation of CHOP-10 during differentiation of 3T3-L1 preadipocytes. / Huang, Haiyan; Lane, M. Daniel; Tang, Qi Qun.
In: Biochemical and Biophysical Research Communications, Vol. 338, No. 2, 16.12.2005, p. 1185-1188.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of serum on the down-regulation of CHOP-10 during differentiation of 3T3-L1 preadipocytes
AU - Huang, Haiyan
AU - Lane, M. Daniel
AU - Tang, Qi Qun
PY - 2005/12/16
Y1 - 2005/12/16
N2 - Hormonal induction of growth-arrested 3T3-L1 preadipocytes in medium containing fetal bovine serum (FBS) triggers a signaling cascade that culminates in adipogenesis. The transcription factor, C/EBPβ, is expressed early in this differentiation program, but lacks DNA-binding activity until much later as the preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Dominant-negative CHOP-10 is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPβ by heterodimerization with its leucine zipper. After a lag period, CHOP-10 undergoes down-regulation releasing C/EBPβ from inhibitory constraint allowing transactivation of the C/EBPα and PPARγ genes, transcription factors required for terminal differentiation. We verify that following induction of differentiation in FBS-containing medium, CHOP-10 undergoes down-regulation and differentiation occurs normally. However, when differentiation is induced in calf serum-containing medium, CHOP-10 is not down-regulated resulting in delayed and incomplete differentiation. Under these conditions the expression of C/EBPα and PPARγ and the accumulation of cytoplasmic triglyceride are attenuated. It appears that a factor(s) present in FBS is required to affect the down-regulation of CHOP-10 necessary for successful terminal differentiation.
AB - Hormonal induction of growth-arrested 3T3-L1 preadipocytes in medium containing fetal bovine serum (FBS) triggers a signaling cascade that culminates in adipogenesis. The transcription factor, C/EBPβ, is expressed early in this differentiation program, but lacks DNA-binding activity until much later as the preadipocytes traverse the G1-S checkpoint of mitotic clonal expansion. Dominant-negative CHOP-10 is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPβ by heterodimerization with its leucine zipper. After a lag period, CHOP-10 undergoes down-regulation releasing C/EBPβ from inhibitory constraint allowing transactivation of the C/EBPα and PPARγ genes, transcription factors required for terminal differentiation. We verify that following induction of differentiation in FBS-containing medium, CHOP-10 undergoes down-regulation and differentiation occurs normally. However, when differentiation is induced in calf serum-containing medium, CHOP-10 is not down-regulated resulting in delayed and incomplete differentiation. Under these conditions the expression of C/EBPα and PPARγ and the accumulation of cytoplasmic triglyceride are attenuated. It appears that a factor(s) present in FBS is required to affect the down-regulation of CHOP-10 necessary for successful terminal differentiation.
KW - Adipocyte differentiation
KW - C/EBPα
KW - C/EBPβ
KW - PPARγ
UR - http://www.scopus.com/inward/record.url?scp=27744469307&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27744469307&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.10.057
DO - 10.1016/j.bbrc.2005.10.057
M3 - Article
C2 - 16256946
AN - SCOPUS:27744469307
VL - 338
SP - 1185
EP - 1188
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -