Effect of recombinant human c-kit receptor ligand on mediator release from human skin mast cells

Michele Columbo, Edward M. Horowitz, Luis M. Botana, Donald W. Macglasha, Bruce S. Bochner, Steven Gillis, Krisztina M. Zsebo, Stephen J. Galli, Lawrence M. Lichtenstein

Research output: Contribution to journalArticle

Abstract

We studied the activity of recombinant human stem cell factor (rhSCF) on the release of mediators from human skin mast cells. High concentrations of rhSCF (1 ng/ml-1 µg/ml) induced a rapid and sustained rise in intracellular Ca2+ levels that was accompanied by release of histamine and prostaglandin D: (PGD:). A brief incubation (10 min) with lower concentrations of rhSCF (0.1 pg/ml-1 ng/ml) enhanced anti-IgE-induced histamine release, but not the release of PGD2. Since some of the effects of rhSCF on mast cell mediator release in vitro occur at concentrations of cytokine similar to those found in the scrum of normal subjects, it is possible that SCF contributes to the modulation of mast cell function under physiological conditions.

Original languageEnglish (US)
Pages (from-to)323-325
Number of pages3
JournalInternational Archives of Allergy and Immunology
Volume99
Issue number2-4
DOIs
StatePublished - Jan 1 1992

Keywords

  • Anti-IgE
  • Histamine
  • Mast cells
  • Prostaglandin D<inf>2</inf>

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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