Effect of protein-hydroxyethylmethacrylate hydrogels on cultured endothelial cells

Barbara Faris, Patricia Mozzicato, Peter Mogayzel, Rocco Ferrera, Louis C. Gerstenfeld, Michael Glembourtt, Joseph S. Makarski, Christian C. Haudenschild, Carl Franzblau

Research output: Contribution to journalArticle

Abstract

The use of protein hydroxy ethylmethacrylate (HEMA) hydrogels to control cell morphology and growth, as well as the synthesis of extracellular matrix components, is described in this communication. HEMA hydrogels prepared with collagen support growth of embryonic lung fibroblasts (IMR-90), as well as bovine aortic and pulmonary artery endothelial cells at a level comparable to the respective cells grown on tissue culture surfaces. On the other hand, HEMA hydrogels prepared with solubilized elastin inhibit the fibroblast growth and prevent both types of endothelial cell cultures from achieving their normal morphology. These morphologically altered endothelial cells resume a normal cobblestone-like appearance when subcultivated from the elastin-HEMA hydrogels to tissue culture plastic. When pulsed with [14C]proline, the procollagens synthesized by the endothelial cells on the different surfaces vary, as shown by immunoprecipitation and polyacrylamide gel electrophoresis. On the standard tissue culture plastic, the confluent cells produce mainly type III procollagen in the medium, whereas those endothelial cells grown on collagen and elastin-HEMA hydrogels synthesize primarily type I procollagen (much like sprouting cells on tissue culture plastic), regardless of their morphology.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalExperimental Cell Research
Volume143
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Hydrogels
Cultured Cells
Endothelial Cells
Elastin
Plastics
Proteins
Collagen
Growth
Fibroblasts
Procollagen
Collagen Type III
Collagen Type I
Immunoprecipitation
Proline
Pulmonary Artery
Extracellular Matrix
Polyacrylamide Gel Electrophoresis
Cell Culture Techniques
poly(ethylmethacrylate)
Lung

ASJC Scopus subject areas

  • Cell Biology

Cite this

Faris, B., Mozzicato, P., Mogayzel, P., Ferrera, R., Gerstenfeld, L. C., Glembourtt, M., ... Franzblau, C. (1983). Effect of protein-hydroxyethylmethacrylate hydrogels on cultured endothelial cells. Experimental Cell Research, 143(1), 15-25. https://doi.org/10.1016/0014-4827(83)90104-0

Effect of protein-hydroxyethylmethacrylate hydrogels on cultured endothelial cells. / Faris, Barbara; Mozzicato, Patricia; Mogayzel, Peter; Ferrera, Rocco; Gerstenfeld, Louis C.; Glembourtt, Michael; Makarski, Joseph S.; Haudenschild, Christian C.; Franzblau, Carl.

In: Experimental Cell Research, Vol. 143, No. 1, 1983, p. 15-25.

Research output: Contribution to journalArticle

Faris, B, Mozzicato, P, Mogayzel, P, Ferrera, R, Gerstenfeld, LC, Glembourtt, M, Makarski, JS, Haudenschild, CC & Franzblau, C 1983, 'Effect of protein-hydroxyethylmethacrylate hydrogels on cultured endothelial cells', Experimental Cell Research, vol. 143, no. 1, pp. 15-25. https://doi.org/10.1016/0014-4827(83)90104-0
Faris, Barbara ; Mozzicato, Patricia ; Mogayzel, Peter ; Ferrera, Rocco ; Gerstenfeld, Louis C. ; Glembourtt, Michael ; Makarski, Joseph S. ; Haudenschild, Christian C. ; Franzblau, Carl. / Effect of protein-hydroxyethylmethacrylate hydrogels on cultured endothelial cells. In: Experimental Cell Research. 1983 ; Vol. 143, No. 1. pp. 15-25.
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