Effect of progesterone on calcium activated potassium currents and intracellular calcium in guinea pig colon myocytes

L. Xu, J. Chen, Baoping Yu, W. Dong, K. Chen, H. Luo, Y. Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: To study the effects of progesterone on contractile activity of smooth muscle strips and on ion currents and intracellular Ca2+ ([Ca2+]i) intensity in single colonie myocytes in guinea pig proximal colons. Methods: Strips and single cells were dissected from female guinea pig proximal colon. Contraction of strips through an isotonic transducer was assessed and the responsible currents to progesterone were recorded with EPC-9 amplifier in nystatin perforated whole-cell configuration. Detection of [Ca 2+]i fluorescence loading fura-2 acetoxymethylester (fura-2/AM) was measured with confocal microscope. Results: Progesterone significantly inhibited contraction of guinea pig colon strips in a dose-dependent pattern. Inhibitory concentration 50 (IC50) of progesterone in longitudinal strips and circular strips was, respectively, 9.7 μM and 1.0 nM. Iberiotoxin (IbTX) partially blocked inhibition of progesterone in both oriented smooth muscle strips. Ca2+ activated K+ (Kca) channel currents recorded by depolarizing pulse protocol were enhanced by progesterone to 138% ± 13% (n = 9, p < 0.01), and to 143% ± 72% (n = 8, p < 0.01) when perfused with 10 μM onapristone. Progesterone reduced L-Ca2+ currents to 67% ± 6% (n = 7, p < 0.01) and had no effect with 5 μM nicardipine in bath solution. [Ca2+]i fluorescence was reduced by progesterone to 75% ± 72% (n = 8, p < 0.01). Conclusions: Progesterone decreases the contraction of colonic smooth muscles by enhancing KCa currents and reducing Ca + influx.

Original languageEnglish (US)
Pages (from-to)475-482
Number of pages8
JournalMethods and Findings in Experimental and Clinical Pharmacology
Volume27
Issue number7
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

Keywords

  • Ca activated K (K) channel
  • Colonic smooth muscle
  • Contraction
  • Intracellular calcium
  • Progesterone

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Effect of progesterone on calcium activated potassium currents and intracellular calcium in guinea pig colon myocytes'. Together they form a unique fingerprint.

Cite this