Effect of pre-loading oral glucosamine HCI/chondroitin sulfate/manganese ascorbate combination on expierimental arthritis in rats

Joel Beren, Susan L. Hill, Marie Diener-West, Noel R. Rose

Research output: Contribution to journalArticlepeer-review

Abstract

The therapeutic effect of a nutritional supplement consisting of a combination of glucosamine hydrochloride (FCHG49), purified sodium chondroitin sulfate (TRH122), and manganese ascorbate (GCM) 3 was investigated in the rat model of collagen-induced autoimmune arthritis (CIA). The GCM compound was mixed with a palatable nutritional paste (Nutri-cal® [NC]). Oral administration of the NC/GCM compound was initiated in 26 rats 10 days before immunization and continued until the day of sacrifice. One group of 12 control rats was given no oral agents; a second group of 12 control rats received NC only. Evaluations included arthritis index (AI) scoring by three independent evaluators, histologic index (HI) scoring of lesions, T-cell proliferation, and serological studies for antibody classes and subclasses. Both the AI and HI criteria showed a statistically significant reduction in the prevalence of CIA in rats pretreated with the NC/GCM (54%) compared to the combined control groups (96%, χ 2 analysis P = 0.001). Rats fed the NC/GCM also exhibited a significant decrease in the severity of autoimmune arthritis in both the AI and HI compared to control Group 2 (immunized-NC) (χ 2 analysis P < 0.05). Histological studies verified the decreased incidence of arthritis in the NC/GCM group compared to control Group 2. GCM treatment failed to alter T-cell proliferation and antibody production to bovine type-II collagen, indicating that its effects are not due to alteration of the antigen-specific immune response.

Original languageEnglish (US)
Pages (from-to)144-151
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Volume226
Issue number2
StatePublished - Feb 22 2001

Keywords

  • Arthritis
  • Glucosamine hydrochloride
  • Sodium chondroitin sulfate

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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