Effect of plasma from patients with liver disease on resistance of red blood cells to lysis by saponin

S. Charache, S. Margolis

Research output: Contribution to journalArticle

Abstract

"Leukocyte counts" of 4 patients with obstructive liver disease, performed with an electronic counter, were > 100,000 per cubic millimeter, while visual counts, performed with 0.1N HCl in a counting chamber, were < 15,000 per cubic millimeter. Red cells in diluted samples of their blood were found to be resistant to lysis by the saponin used to prepare samples for electronic counting. Resistance to lysis was eliminated by washing the red cells and was produced by suspension of normal red cells in patient's plasma. Resistance, which was conferred by the plasma lipoproteins of density 1.006 to 1.063, appeared to be due to the unusually high concentration of nonesterified cholesterol in this lipoprotein fraction.

Original languageEnglish (US)
Pages (from-to)951-955
Number of pages5
JournalThe Journal of laboratory and clinical medicine
Volume73
Issue number6
StatePublished - Jun 1 1969

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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