Effect of pentoxifylline on vascular endothelial growth factor C and flk-1 expression on endometrial implants in the rat endometriosis model

Nikos F. Vlahos, Odysseas Gregoriou, Aikaterini Deliveliotou, Despoina Perrea, Athanasios Vlachos, Yulian Zhao, Joseph Lai, George Creatsas

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the effects of pentoxifylline, on vascular endothelial growth factor (VEGF)-C and flk-1 expression in the rat endometriosis model. Design: Prospective, randomized, placebo-controlled study. Setting: Academic institution. Animal(s): Twenty Wistar rats with surgically induced endometriosis. Intervention(s): Animals were evaluated after surgical induction of endometriosis and random allocation to a group that received pentoxifylline and a control group that received NaCl 0.9%, for 3 weeks. At the end of the treatment period the animals were killed and the implants evaluated macroscopically as well as by immunohistochemistry. Main Outcome Measure(s): Morphologic changes of the endometriotic implants; and evaluation of VEGF-C and flk-1 expression by a semiquantitative analysis (HSCORE) for the intensity of immunohistochemical reactivity. Result(s): A significant reduction was observed in the mean volume of the endometriotic implants per animal in the treatment group as compared with the control group. There was a significant reduction not only in the mean volume of implants per animal but also in the mean number of implants per animal after treatment. By immunohistochemical evaluation (HSCORE), there was a significant reduction in VEGF-C expression after treatment in all areas examined. A significant reduction of flk-1 expression was also noted in the glandular compartment after treatment but not in the epithelial surface or stroma. Conclusion(s): Pentoxifylline may cause suppression of endometriotic lesions by suppressing angiogenesis through VEGF-C and flk-1 expression.

Original languageEnglish (US)
Pages (from-to)1316-1323
Number of pages8
JournalFertility and sterility
Volume93
Issue number4
DOIs
StatePublished - Mar 1 2010

Keywords

  • Endometriosis
  • VEGF
  • angiogenesis
  • flk-1
  • pentoxifylline
  • rat

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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