TY - JOUR
T1 - Effect of organ site on nuclear matrix protein composition
AU - Replogle-Schwab, Tracy S.
AU - Getzenberg, Robert H.
AU - Donat, Terry L.
AU - Pienta, Kenneth J.
PY - 1996/7
Y1 - 1996/7
N2 - The nuclear matrix has been linked to several important cellular functions within cells, such as DNA organization and replication, as well as regulation of gene expression. It has been reported that the nuclear matrix protein composition is altered in cells grown on different extracellular matrices in vitro. This study examined the nuclear matrix protein composition of tumors produced by MAT-LyLu (MLL) rat prostate tumor cells implanted at different organ sites within the rat. When high resolution two-dimensional gels were utilized to compare nuclear matrix protein composition to the prostate orthotopic tumor, it was found that there were distinct protein differences depending upon where the tumor grew. In particular, there were 14 proteins found in the lung, six proteins found in intramuscular, 17 proteins is the heart, and five proteins in the tail vein tumor tissue that were not present in the prostate orthotopic tumor tissue. Therefore, this study adds evidence to support that the nuclear matrix composition of a cell is dependent, at least in part, by the extracellular matrix and/or different cellular environments and may have a role in site-specific differences in tumor properties.
AB - The nuclear matrix has been linked to several important cellular functions within cells, such as DNA organization and replication, as well as regulation of gene expression. It has been reported that the nuclear matrix protein composition is altered in cells grown on different extracellular matrices in vitro. This study examined the nuclear matrix protein composition of tumors produced by MAT-LyLu (MLL) rat prostate tumor cells implanted at different organ sites within the rat. When high resolution two-dimensional gels were utilized to compare nuclear matrix protein composition to the prostate orthotopic tumor, it was found that there were distinct protein differences depending upon where the tumor grew. In particular, there were 14 proteins found in the lung, six proteins found in intramuscular, 17 proteins is the heart, and five proteins in the tail vein tumor tissue that were not present in the prostate orthotopic tumor tissue. Therefore, this study adds evidence to support that the nuclear matrix composition of a cell is dependent, at least in part, by the extracellular matrix and/or different cellular environments and may have a role in site-specific differences in tumor properties.
KW - MAT-LyLu (MLL)
KW - cancer
KW - extracellular matrix
KW - gene expression
KW - orthotopic
KW - two-dimensional electrophoresis
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U2 - 10.1002/(SICI)1097-4644(199607)62:1<132::AID-JCB14>3.0.CO;2-P
DO - 10.1002/(SICI)1097-4644(199607)62:1<132::AID-JCB14>3.0.CO;2-P
M3 - Article
C2 - 8836882
AN - SCOPUS:0029797736
SN - 0730-2312
VL - 62
SP - 132
EP - 141
JO - Journal of cellular biochemistry
JF - Journal of cellular biochemistry
IS - 1
ER -