Effect of nanoparticle conjugation on gene silencing by RNA interference

Neetu Singh, Amit Agrawal, Anthony Kar Lun Leung, Phillip A. Sharp, Sangeeta N. Bhatia

Research output: Contribution to journalArticle

Abstract

RNA interference (RNAi) is a cellular process whereby the silencing of a particular gene is mediated by short RNAs (siRNAs). Although siRNAs have great therapeutic potential, cellular delivery has been a challenge. Nanoparticle-siRNA conjugates have emerged as potential delivery vehicles; however, reports describing the effects of nanoparticle conjugation on RISC incorporation and subsequent gene silencing have been mixed. In this report, we have systematically evaluated the effect of siRNA coupling strategies using a model nanoparticle system with varying conjugation schemes. We show that the accessibility of the siRNA linked to the nanoparticle and the lability of the cross-linker are critical for efficient gene knockdown.

Original languageEnglish (US)
Pages (from-to)8241-8243
Number of pages3
JournalJournal of the American Chemical Society
Volume132
Issue number24
DOIs
StatePublished - Jun 23 2010
Externally publishedYes

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Gene Silencing
RNA Interference
RNA
Nanoparticles
Genes
Small Interfering RNA
Gene Knockdown Techniques
Reduced instruction set computing
Therapeutics

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Effect of nanoparticle conjugation on gene silencing by RNA interference. / Singh, Neetu; Agrawal, Amit; Leung, Anthony Kar Lun; Sharp, Phillip A.; Bhatia, Sangeeta N.

In: Journal of the American Chemical Society, Vol. 132, No. 24, 23.06.2010, p. 8241-8243.

Research output: Contribution to journalArticle

Singh, Neetu ; Agrawal, Amit ; Leung, Anthony Kar Lun ; Sharp, Phillip A. ; Bhatia, Sangeeta N. / Effect of nanoparticle conjugation on gene silencing by RNA interference. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 24. pp. 8241-8243.
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