Effect of MOG sensitization on somatosensory evoked potential in Lewis rats

Angelo Homayoun All, Piotr Walczak, Gracee Agrawal, Michael Gorelik, Christopher Lee, Nitish V Thakor, Jeff W Bulte, Douglas A. Kerr

Research output: Contribution to journalArticle

Abstract

Myelin oligodendrocyte glycoprotein (MOG) is commonly used as an immunogen to induce an immune response against endogenous myelin, thereby modeling multiple sclerosis in rodents. When MOG is combined with complete Freund's adjuvant (CFA), multifocal, multiphasic disease ensues; whereas when MOG is combined with incomplete Freund's adjuvant (IFA), clinical disease is usually absent. MOG-IFA immunized animals can be induced to have neurological disease after intraspinal injections of cytokines and ethidium bromide (EtBr). In this study, we investigated whether MOG-IFA immunized rats exhibited subclinical injury as defined by somatosensory evoked potential (SEP) recordings. The titration of anti-MOG-125 antibodies showed robust peripheral mounting of immune response against myelin in MOG-immunized rats. However the SEP measures showed no significant change over time. Upon injecting cytokine-EtBr in the spinal cord after MOG sensitization, the SEP recordings showed reduced amplitude and prolonged latency, suggestive of axonal injury and demyelination in the dorsal column, respectively. These findings were later confirmed using T2-weighted MRI and histological hematoxylin-eosin stain of the spinal cord. This report establishes that MOG-IFA immunization alone does not alter neuronal conduction in SEP-related neural-pathways and that longitudinal in-vivo SEP recordings provide a sensitive read-out for focal myelitis (MOG-IFA and intraspinal cytokine-EtBr) in rats.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalJournal of the Neurological Sciences
Volume284
Issue number1-2
DOIs
StatePublished - Sep 15 2009

Fingerprint

Myelin-Oligodendrocyte Glycoprotein
Somatosensory Evoked Potentials
Ethidium
Cytokines
Myelin Sheath
Spinal Cord
Myelitis
Neural Pathways
Spinal Injections
Freund's Adjuvant
Wounds and Injuries
Demyelinating Diseases
Hematoxylin
Eosine Yellowish-(YS)
Multiple Sclerosis
Rodentia
Immunization
Coloring Agents
incomplete Freund's adjuvant

Keywords

  • Cytokine
  • Ethidium bromide
  • Freund's adjuvant
  • Myelin oligodendrocyte glycoprotein (MOG)
  • Sensitization/immunization
  • Somatosensory evoked potential (SEP)

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Effect of MOG sensitization on somatosensory evoked potential in Lewis rats. / All, Angelo Homayoun; Walczak, Piotr; Agrawal, Gracee; Gorelik, Michael; Lee, Christopher; Thakor, Nitish V; Bulte, Jeff W; Kerr, Douglas A.

In: Journal of the Neurological Sciences, Vol. 284, No. 1-2, 15.09.2009, p. 81-89.

Research output: Contribution to journalArticle

All, Angelo Homayoun ; Walczak, Piotr ; Agrawal, Gracee ; Gorelik, Michael ; Lee, Christopher ; Thakor, Nitish V ; Bulte, Jeff W ; Kerr, Douglas A. / Effect of MOG sensitization on somatosensory evoked potential in Lewis rats. In: Journal of the Neurological Sciences. 2009 ; Vol. 284, No. 1-2. pp. 81-89.
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