TY - JOUR
T1 - Effect of MOG sensitization on somatosensory evoked potential in Lewis rats
AU - All, Angelo H.
AU - Walczak, Piotr
AU - Agrawal, Gracee
AU - Gorelik, Michael
AU - Lee, Christopher
AU - Thakor, Nitish V.
AU - Bulte, Jeff W.M.
AU - Kerr, Douglas A.
N1 - Funding Information:
The study was supported by the Johns Hopkins Project RESTORE fund (Transverse Myelitis Research Project) and the Maryland Stem Cell Research Fund 2007-MSCRFII-0159-00. A part of this study was also supported under the grants RO1 NS045062, NMSS RG 3630 and National Multiple Sclerosis Society Grant (PP1491).
PY - 2009/9/15
Y1 - 2009/9/15
N2 - Myelin oligodendrocyte glycoprotein (MOG) is commonly used as an immunogen to induce an immune response against endogenous myelin, thereby modeling multiple sclerosis in rodents. When MOG is combined with complete Freund's adjuvant (CFA), multifocal, multiphasic disease ensues; whereas when MOG is combined with incomplete Freund's adjuvant (IFA), clinical disease is usually absent. MOG-IFA immunized animals can be induced to have neurological disease after intraspinal injections of cytokines and ethidium bromide (EtBr). In this study, we investigated whether MOG-IFA immunized rats exhibited subclinical injury as defined by somatosensory evoked potential (SEP) recordings. The titration of anti-MOG-125 antibodies showed robust peripheral mounting of immune response against myelin in MOG-immunized rats. However the SEP measures showed no significant change over time. Upon injecting cytokine-EtBr in the spinal cord after MOG sensitization, the SEP recordings showed reduced amplitude and prolonged latency, suggestive of axonal injury and demyelination in the dorsal column, respectively. These findings were later confirmed using T2-weighted MRI and histological hematoxylin-eosin stain of the spinal cord. This report establishes that MOG-IFA immunization alone does not alter neuronal conduction in SEP-related neural-pathways and that longitudinal in-vivo SEP recordings provide a sensitive read-out for focal myelitis (MOG-IFA and intraspinal cytokine-EtBr) in rats.
AB - Myelin oligodendrocyte glycoprotein (MOG) is commonly used as an immunogen to induce an immune response against endogenous myelin, thereby modeling multiple sclerosis in rodents. When MOG is combined with complete Freund's adjuvant (CFA), multifocal, multiphasic disease ensues; whereas when MOG is combined with incomplete Freund's adjuvant (IFA), clinical disease is usually absent. MOG-IFA immunized animals can be induced to have neurological disease after intraspinal injections of cytokines and ethidium bromide (EtBr). In this study, we investigated whether MOG-IFA immunized rats exhibited subclinical injury as defined by somatosensory evoked potential (SEP) recordings. The titration of anti-MOG-125 antibodies showed robust peripheral mounting of immune response against myelin in MOG-immunized rats. However the SEP measures showed no significant change over time. Upon injecting cytokine-EtBr in the spinal cord after MOG sensitization, the SEP recordings showed reduced amplitude and prolonged latency, suggestive of axonal injury and demyelination in the dorsal column, respectively. These findings were later confirmed using T2-weighted MRI and histological hematoxylin-eosin stain of the spinal cord. This report establishes that MOG-IFA immunization alone does not alter neuronal conduction in SEP-related neural-pathways and that longitudinal in-vivo SEP recordings provide a sensitive read-out for focal myelitis (MOG-IFA and intraspinal cytokine-EtBr) in rats.
KW - Cytokine
KW - Ethidium bromide
KW - Freund's adjuvant
KW - Myelin oligodendrocyte glycoprotein (MOG)
KW - Sensitization/immunization
KW - Somatosensory evoked potential (SEP)
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U2 - 10.1016/j.jns.2009.04.025
DO - 10.1016/j.jns.2009.04.025
M3 - Article
C2 - 19423134
AN - SCOPUS:67849088555
SN - 0022-510X
VL - 284
SP - 81
EP - 89
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
IS - 1-2
ER -