Effect of middle cerebral artery occlusion on mRNA expression for the sodium-coupled vitamin C transporter SVCT2 in rat brain

Urs V. Berger, Xi Chun M. Lu, Weilin Liu, Zhaocheng Tang, Barbara S. Slusher, Matthias A. Hediger

Research output: Contribution to journalArticlepeer-review

Abstract

The sodium-vitamin C co-transporter SVCT2 is primarily responsible for the accumulation of the important antioxidant ascorbate into brain cells. In vitro studies have demonstrated strong expression of this transporter in cultured astrocytes, whereas in situ hybridization analysis has so far detected SVCT2 only in neurons. In the present study, we examined the response of SVCT2 mRNA expression in the brain to focal ischemia induced for 2 h by unilateral middle cerebral artery occlusion. The mRNA expression patterns of SVCT2 and the glutamate-activated immediate early gene Arc were investigated at 2 and 22 h after ischemia. SVCT2 and Arc mRNA expression was lost in the ischemic core at both time points. In areas outside the core, Arc was strongly up-regulated, primarily at 2 h, whereas SVCT2 showed an increase at 2 and 22 h. SVCT2 expression was increased in neurons as well as in astrocytes, providing the first evidence for SVCT2 expression in astrocytes in situ. These findings underscore the importance of ascorbate as a neuroprotective agent and may have implications for therapeutic strategies. In addition, the increase of SVCT2 in astrocytes after ischemia suggests that cultured astrocytes are exposed to chronic oxidative stress.

Original languageEnglish (US)
Pages (from-to)896-906
Number of pages11
JournalJournal of Neurochemistry
Volume86
Issue number4
DOIs
StatePublished - Aug 2003
Externally publishedYes

Keywords

  • Ascorbate
  • Astrocytes
  • Astrocytosis
  • Ischemia
  • Oxidative stress
  • SVCT2

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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