To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3′-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 ± 0.62) × 10-9 cm2/s with (77.0 ± 2.1)% recovery, and on the budding polar body we found D = (7.05 ± 0.75) × 10-9 cm2/s with (84.7 ± 1.3)% recovery. We thus find only slight differences in diffusion in the two regions.
|Original language||English (US)|
|Number of pages||3|
|State||Published - 1982|
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