Effect of Microvilli on Lateral Diffusion Measurements Made by the Fluorescence Photobleaching Recovery Technique

David E. Wolf, Alan H. Handyside, Michael Edidin

Research output: Contribution to journalArticlepeer-review

Abstract

To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3′-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 ± 0.62) × 10-9 cm2/s with (77.0 ± 2.1)% recovery, and on the budding polar body we found D = (7.05 ± 0.75) × 10-9 cm2/s with (84.7 ± 1.3)% recovery. We thus find only slight differences in diffusion in the two regions.

Original languageEnglish (US)
Pages (from-to)295-297
Number of pages3
JournalBiophysical journal
Volume38
Issue number3
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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