Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse

Lindsay S. Cahill, Clare L. Whitehead, Sebastian R. Hobson, Greg Stortz, John C. Kingdom, Ahmet Baschat, Kellie E. Murphy, Lena Serghides, Christopher K. Macgowan, John G. Sled

Research output: Contribution to journalArticle

Abstract

Antenatal corticosteroids are often administered to women at risk of preterm birth to accelerate fetal lung development; however, there is evidence that this treatment may adversely affect placental function in some fetuses. Our group has recently demonstrated that wave reflections in the umbilical artery (UA), measured using high-frequency ultrasound, are sensitive to placental vascular abnormalities. In the present study, we used this approach to investigate the effect of maternal administration of betamethasone, a clinically relevant corticosteroid, on the feto-placental vasculature of the mouse. Fetuses were assessed at embryonic day (E)15.5 and E17.5 in C57BL6/J mice. At both gestational ages, the UA diameter, UA blood flow, and the wave reflection coefficient were significantly elevated in the betamethasone-treated mice compared to vehicle-treated controls. These observations support the interpretation that placental vascular resistance dropped with betamethasone treatment to an extent that could not be explained by vasodilation of the UA alone. Consistent with clinical studies, the effect of betamethasone on UA end-diastolic velocity was heterogeneous. Our results suggest that UA wave reflections are more sensitive to acute changes in placental vascular resistance compared with the UA pulsatility index, and this technique may have clinical application to identify a favorable placental vascular response to fetal therapies such as antenatal corticosteroids, where the fetal heart rate is likely to vary.

Original languageEnglish (US)
Pages (from-to)823-831
Number of pages9
JournalBiology of Reproduction
Volume101
Issue number4
DOIs
StatePublished - Oct 25 2019

Fingerprint

Betamethasone
Umbilical Arteries
Vascular Resistance
Mothers
Adrenal Cortex Hormones
Blood Vessels
Fetus
Fetal Therapies
Fetal Heart Rate
Premature Birth
Fetal Development
Vasodilation
Gestational Age
Lung
Therapeutics

Keywords

  • corticosteroids
  • mouse
  • placenta
  • ultrasound
  • umbilical artery
  • wave reflection

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Cahill, L. S., Whitehead, C. L., Hobson, S. R., Stortz, G., Kingdom, J. C., Baschat, A., ... Sled, J. G. (2019). Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse. Biology of Reproduction, 101(4), 823-831. https://doi.org/10.1093/biolre/ioz128

Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse. / Cahill, Lindsay S.; Whitehead, Clare L.; Hobson, Sebastian R.; Stortz, Greg; Kingdom, John C.; Baschat, Ahmet; Murphy, Kellie E.; Serghides, Lena; Macgowan, Christopher K.; Sled, John G.

In: Biology of Reproduction, Vol. 101, No. 4, 25.10.2019, p. 823-831.

Research output: Contribution to journalArticle

Cahill, LS, Whitehead, CL, Hobson, SR, Stortz, G, Kingdom, JC, Baschat, A, Murphy, KE, Serghides, L, Macgowan, CK & Sled, JG 2019, 'Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse', Biology of Reproduction, vol. 101, no. 4, pp. 823-831. https://doi.org/10.1093/biolre/ioz128
Cahill, Lindsay S. ; Whitehead, Clare L. ; Hobson, Sebastian R. ; Stortz, Greg ; Kingdom, John C. ; Baschat, Ahmet ; Murphy, Kellie E. ; Serghides, Lena ; Macgowan, Christopher K. ; Sled, John G. / Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse. In: Biology of Reproduction. 2019 ; Vol. 101, No. 4. pp. 823-831.
@article{fb018f28d5f94aad9a014ca23a9891eb,
title = "Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse",
abstract = "Antenatal corticosteroids are often administered to women at risk of preterm birth to accelerate fetal lung development; however, there is evidence that this treatment may adversely affect placental function in some fetuses. Our group has recently demonstrated that wave reflections in the umbilical artery (UA), measured using high-frequency ultrasound, are sensitive to placental vascular abnormalities. In the present study, we used this approach to investigate the effect of maternal administration of betamethasone, a clinically relevant corticosteroid, on the feto-placental vasculature of the mouse. Fetuses were assessed at embryonic day (E)15.5 and E17.5 in C57BL6/J mice. At both gestational ages, the UA diameter, UA blood flow, and the wave reflection coefficient were significantly elevated in the betamethasone-treated mice compared to vehicle-treated controls. These observations support the interpretation that placental vascular resistance dropped with betamethasone treatment to an extent that could not be explained by vasodilation of the UA alone. Consistent with clinical studies, the effect of betamethasone on UA end-diastolic velocity was heterogeneous. Our results suggest that UA wave reflections are more sensitive to acute changes in placental vascular resistance compared with the UA pulsatility index, and this technique may have clinical application to identify a favorable placental vascular response to fetal therapies such as antenatal corticosteroids, where the fetal heart rate is likely to vary.",
keywords = "corticosteroids, mouse, placenta, ultrasound, umbilical artery, wave reflection",
author = "Cahill, {Lindsay S.} and Whitehead, {Clare L.} and Hobson, {Sebastian R.} and Greg Stortz and Kingdom, {John C.} and Ahmet Baschat and Murphy, {Kellie E.} and Lena Serghides and Macgowan, {Christopher K.} and Sled, {John G.}",
year = "2019",
month = "10",
day = "25",
doi = "10.1093/biolre/ioz128",
language = "English (US)",
volume = "101",
pages = "823--831",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Effect of maternal betamethasone administration on feto-placental vascular resistance in the mouse

AU - Cahill, Lindsay S.

AU - Whitehead, Clare L.

AU - Hobson, Sebastian R.

AU - Stortz, Greg

AU - Kingdom, John C.

AU - Baschat, Ahmet

AU - Murphy, Kellie E.

AU - Serghides, Lena

AU - Macgowan, Christopher K.

AU - Sled, John G.

PY - 2019/10/25

Y1 - 2019/10/25

N2 - Antenatal corticosteroids are often administered to women at risk of preterm birth to accelerate fetal lung development; however, there is evidence that this treatment may adversely affect placental function in some fetuses. Our group has recently demonstrated that wave reflections in the umbilical artery (UA), measured using high-frequency ultrasound, are sensitive to placental vascular abnormalities. In the present study, we used this approach to investigate the effect of maternal administration of betamethasone, a clinically relevant corticosteroid, on the feto-placental vasculature of the mouse. Fetuses were assessed at embryonic day (E)15.5 and E17.5 in C57BL6/J mice. At both gestational ages, the UA diameter, UA blood flow, and the wave reflection coefficient were significantly elevated in the betamethasone-treated mice compared to vehicle-treated controls. These observations support the interpretation that placental vascular resistance dropped with betamethasone treatment to an extent that could not be explained by vasodilation of the UA alone. Consistent with clinical studies, the effect of betamethasone on UA end-diastolic velocity was heterogeneous. Our results suggest that UA wave reflections are more sensitive to acute changes in placental vascular resistance compared with the UA pulsatility index, and this technique may have clinical application to identify a favorable placental vascular response to fetal therapies such as antenatal corticosteroids, where the fetal heart rate is likely to vary.

AB - Antenatal corticosteroids are often administered to women at risk of preterm birth to accelerate fetal lung development; however, there is evidence that this treatment may adversely affect placental function in some fetuses. Our group has recently demonstrated that wave reflections in the umbilical artery (UA), measured using high-frequency ultrasound, are sensitive to placental vascular abnormalities. In the present study, we used this approach to investigate the effect of maternal administration of betamethasone, a clinically relevant corticosteroid, on the feto-placental vasculature of the mouse. Fetuses were assessed at embryonic day (E)15.5 and E17.5 in C57BL6/J mice. At both gestational ages, the UA diameter, UA blood flow, and the wave reflection coefficient were significantly elevated in the betamethasone-treated mice compared to vehicle-treated controls. These observations support the interpretation that placental vascular resistance dropped with betamethasone treatment to an extent that could not be explained by vasodilation of the UA alone. Consistent with clinical studies, the effect of betamethasone on UA end-diastolic velocity was heterogeneous. Our results suggest that UA wave reflections are more sensitive to acute changes in placental vascular resistance compared with the UA pulsatility index, and this technique may have clinical application to identify a favorable placental vascular response to fetal therapies such as antenatal corticosteroids, where the fetal heart rate is likely to vary.

KW - corticosteroids

KW - mouse

KW - placenta

KW - ultrasound

KW - umbilical artery

KW - wave reflection

UR - http://www.scopus.com/inward/record.url?scp=85075814610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075814610&partnerID=8YFLogxK

U2 - 10.1093/biolre/ioz128

DO - 10.1093/biolre/ioz128

M3 - Article

C2 - 31318405

AN - SCOPUS:85075814610

VL - 101

SP - 823

EP - 831

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -