Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation

Yulian Zhao, Howard A Zacur, Chris Cheadle, Ning Ning, Jinshui Fan, Nikos F. Vlahos

Research output: Contribution to journalArticle

Abstract

Background: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles.Methods: The study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3-5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel.Results: A total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p <0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3-5 days after retrieval. During the peri-implantation period (3-5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group.Conclusion: Luteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes.

Original languageEnglish (US)
Article number72
JournalReproductive Biology and Endocrinology
Volume10
DOIs
StatePublished - Sep 6 2012

Fingerprint

Ovulation Induction
Luteal Phase
MicroRNAs
Corpus Luteum
Uterus
Progesterone
Steroids
Oocyte Retrieval
Biopsy
Self-Help Groups
Research Ethics Committees
Microarray Analysis
Oocytes
Reproduction
Cell Differentiation
Estradiol
Up-Regulation
Down-Regulation
Hormones
RNA

Keywords

  • Luteal phase support
  • Microarray
  • MicroRNA
  • Ovarian stimulation

ASJC Scopus subject areas

  • Developmental Biology
  • Endocrinology
  • Reproductive Medicine

Cite this

Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation. / Zhao, Yulian; Zacur, Howard A; Cheadle, Chris; Ning, Ning; Fan, Jinshui; Vlahos, Nikos F.

In: Reproductive Biology and Endocrinology, Vol. 10, 72, 06.09.2012.

Research output: Contribution to journalArticle

@article{fa38d45bb717472a80df27bd1c36b954,
title = "Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation",
abstract = "Background: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles.Methods: The study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3-5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel.Results: A total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p <0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3-5 days after retrieval. During the peri-implantation period (3-5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group.Conclusion: Luteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes.",
keywords = "Luteal phase support, Microarray, MicroRNA, Ovarian stimulation",
author = "Yulian Zhao and Zacur, {Howard A} and Chris Cheadle and Ning Ning and Jinshui Fan and Vlahos, {Nikos F.}",
year = "2012",
month = "9",
day = "6",
doi = "10.1186/1477-7827-10-72",
language = "English (US)",
volume = "10",
journal = "Reproductive Biology and Endocrinology",
issn = "1477-7827",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation

AU - Zhao, Yulian

AU - Zacur, Howard A

AU - Cheadle, Chris

AU - Ning, Ning

AU - Fan, Jinshui

AU - Vlahos, Nikos F.

PY - 2012/9/6

Y1 - 2012/9/6

N2 - Background: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles.Methods: The study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3-5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel.Results: A total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p <0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3-5 days after retrieval. During the peri-implantation period (3-5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group.Conclusion: Luteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes.

AB - Background: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles.Methods: The study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3-5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel.Results: A total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p <0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3-5 days after retrieval. During the peri-implantation period (3-5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group.Conclusion: Luteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes.

KW - Luteal phase support

KW - Microarray

KW - MicroRNA

KW - Ovarian stimulation

UR - http://www.scopus.com/inward/record.url?scp=84865760054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865760054&partnerID=8YFLogxK

U2 - 10.1186/1477-7827-10-72

DO - 10.1186/1477-7827-10-72

M3 - Article

VL - 10

JO - Reproductive Biology and Endocrinology

JF - Reproductive Biology and Endocrinology

SN - 1477-7827

M1 - 72

ER -