Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model.

Stephen J. Thomas, Joseph J. Sarver, Sarah M. Yannascoli, Jennica J. Tucker, John D. Kelly, Rexford S Ahima, Mary F. Barbe, Louis J. Soslowsky

Research output: Contribution to journalArticle

Abstract

Recently, diabetes has been linked to rotator cuff disease and adhesive capsulitis, conditions with increased stiffness and inflammation. Unfortunately, limited research exists examining how hyperglycemia affects the native shoulder (tendon and capsule) properties. Therefore, the objectives of this study were to compare shoulder joint mechanics, tendon properties (mechanics and immunohistochemistry), and capsule of healthy control and hyperglycemic rats 8 weeks following induction of hyperglycemia with a submaximal dose of streptozotocin (STZ). Eighteen rats were injected with STZ to induce hyperglycemia or citrate buffer (control) and underwent normal cage activity for 8 weeks. Passive joint mechanics demonstrated significantly less external rotation in the hyperglycemic group compared to controls, with no other group differences. Tendon mechanical properties (stiffness and modulus) were not significantly different between groups at both the insertion site and mid-substance. Immunohistochemistry staining of the tendon and capsule demonstrated significantly increased interleukin 1-beta (IL1-β) and advanced glycated end-products (AGE) staining localized to the insertion and mid-substance of the tendon but not the capsule. In addition, tumor necrosis factor alpha (TNF-α) staining was significantly increased in the superior capsule but not the supraspinatus tendon. This study demonstrates that isolated hypergylcemia does not diminish shoulder mechanical properties but does induce a chronic inflammatory response.

Original languageEnglish (US)
Pages (from-to)1464-1470
Number of pages7
JournalJournal of Orthopaedic Research
Volume32
Issue number11
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Shoulder Joint
Hyperglycemia
Tendons
Capsules
Mechanics
Rotator Cuff
Streptozocin
Staining and Labeling
Immunohistochemistry
Bursitis
Interleukin-1beta
Citric Acid
Buffers
Tumor Necrosis Factor-alpha
Joints
Inflammation
Research

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Thomas, S. J., Sarver, J. J., Yannascoli, S. M., Tucker, J. J., Kelly, J. D., Ahima, R. S., ... Soslowsky, L. J. (2014). Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model. Journal of Orthopaedic Research, 32(11), 1464-1470. https://doi.org/10.1002/jor.22695

Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model. / Thomas, Stephen J.; Sarver, Joseph J.; Yannascoli, Sarah M.; Tucker, Jennica J.; Kelly, John D.; Ahima, Rexford S; Barbe, Mary F.; Soslowsky, Louis J.

In: Journal of Orthopaedic Research, Vol. 32, No. 11, 2014, p. 1464-1470.

Research output: Contribution to journalArticle

Thomas, SJ, Sarver, JJ, Yannascoli, SM, Tucker, JJ, Kelly, JD, Ahima, RS, Barbe, MF & Soslowsky, LJ 2014, 'Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model.', Journal of Orthopaedic Research, vol. 32, no. 11, pp. 1464-1470. https://doi.org/10.1002/jor.22695
Thomas, Stephen J. ; Sarver, Joseph J. ; Yannascoli, Sarah M. ; Tucker, Jennica J. ; Kelly, John D. ; Ahima, Rexford S ; Barbe, Mary F. ; Soslowsky, Louis J. / Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model. In: Journal of Orthopaedic Research. 2014 ; Vol. 32, No. 11. pp. 1464-1470.
@article{7aba8554c0de4e948ed7c779a8438f86,
title = "Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model.",
abstract = "Recently, diabetes has been linked to rotator cuff disease and adhesive capsulitis, conditions with increased stiffness and inflammation. Unfortunately, limited research exists examining how hyperglycemia affects the native shoulder (tendon and capsule) properties. Therefore, the objectives of this study were to compare shoulder joint mechanics, tendon properties (mechanics and immunohistochemistry), and capsule of healthy control and hyperglycemic rats 8 weeks following induction of hyperglycemia with a submaximal dose of streptozotocin (STZ). Eighteen rats were injected with STZ to induce hyperglycemia or citrate buffer (control) and underwent normal cage activity for 8 weeks. Passive joint mechanics demonstrated significantly less external rotation in the hyperglycemic group compared to controls, with no other group differences. Tendon mechanical properties (stiffness and modulus) were not significantly different between groups at both the insertion site and mid-substance. Immunohistochemistry staining of the tendon and capsule demonstrated significantly increased interleukin 1-beta (IL1-β) and advanced glycated end-products (AGE) staining localized to the insertion and mid-substance of the tendon but not the capsule. In addition, tumor necrosis factor alpha (TNF-α) staining was significantly increased in the superior capsule but not the supraspinatus tendon. This study demonstrates that isolated hypergylcemia does not diminish shoulder mechanical properties but does induce a chronic inflammatory response.",
author = "Thomas, {Stephen J.} and Sarver, {Joseph J.} and Yannascoli, {Sarah M.} and Tucker, {Jennica J.} and Kelly, {John D.} and Ahima, {Rexford S} and Barbe, {Mary F.} and Soslowsky, {Louis J.}",
year = "2014",
doi = "10.1002/jor.22695",
language = "English (US)",
volume = "32",
pages = "1464--1470",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model.

AU - Thomas, Stephen J.

AU - Sarver, Joseph J.

AU - Yannascoli, Sarah M.

AU - Tucker, Jennica J.

AU - Kelly, John D.

AU - Ahima, Rexford S

AU - Barbe, Mary F.

AU - Soslowsky, Louis J.

PY - 2014

Y1 - 2014

N2 - Recently, diabetes has been linked to rotator cuff disease and adhesive capsulitis, conditions with increased stiffness and inflammation. Unfortunately, limited research exists examining how hyperglycemia affects the native shoulder (tendon and capsule) properties. Therefore, the objectives of this study were to compare shoulder joint mechanics, tendon properties (mechanics and immunohistochemistry), and capsule of healthy control and hyperglycemic rats 8 weeks following induction of hyperglycemia with a submaximal dose of streptozotocin (STZ). Eighteen rats were injected with STZ to induce hyperglycemia or citrate buffer (control) and underwent normal cage activity for 8 weeks. Passive joint mechanics demonstrated significantly less external rotation in the hyperglycemic group compared to controls, with no other group differences. Tendon mechanical properties (stiffness and modulus) were not significantly different between groups at both the insertion site and mid-substance. Immunohistochemistry staining of the tendon and capsule demonstrated significantly increased interleukin 1-beta (IL1-β) and advanced glycated end-products (AGE) staining localized to the insertion and mid-substance of the tendon but not the capsule. In addition, tumor necrosis factor alpha (TNF-α) staining was significantly increased in the superior capsule but not the supraspinatus tendon. This study demonstrates that isolated hypergylcemia does not diminish shoulder mechanical properties but does induce a chronic inflammatory response.

AB - Recently, diabetes has been linked to rotator cuff disease and adhesive capsulitis, conditions with increased stiffness and inflammation. Unfortunately, limited research exists examining how hyperglycemia affects the native shoulder (tendon and capsule) properties. Therefore, the objectives of this study were to compare shoulder joint mechanics, tendon properties (mechanics and immunohistochemistry), and capsule of healthy control and hyperglycemic rats 8 weeks following induction of hyperglycemia with a submaximal dose of streptozotocin (STZ). Eighteen rats were injected with STZ to induce hyperglycemia or citrate buffer (control) and underwent normal cage activity for 8 weeks. Passive joint mechanics demonstrated significantly less external rotation in the hyperglycemic group compared to controls, with no other group differences. Tendon mechanical properties (stiffness and modulus) were not significantly different between groups at both the insertion site and mid-substance. Immunohistochemistry staining of the tendon and capsule demonstrated significantly increased interleukin 1-beta (IL1-β) and advanced glycated end-products (AGE) staining localized to the insertion and mid-substance of the tendon but not the capsule. In addition, tumor necrosis factor alpha (TNF-α) staining was significantly increased in the superior capsule but not the supraspinatus tendon. This study demonstrates that isolated hypergylcemia does not diminish shoulder mechanical properties but does induce a chronic inflammatory response.

UR - http://www.scopus.com/inward/record.url?scp=84910020884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910020884&partnerID=8YFLogxK

U2 - 10.1002/jor.22695

DO - 10.1002/jor.22695

M3 - Article

C2 - 25070655

AN - SCOPUS:84910020884

VL - 32

SP - 1464

EP - 1470

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 11

ER -