Effect of irradiation on vascularization of corneas grafted onto chorioallantoic membranes

H. J. Glatt, M. T. Vu, P. C. Burger, G. K. Klintworth

Research output: Contribution to journalArticlepeer-review

Abstract

Several studies have shown that total body irradiation decreases the angiogenic response to corneal cauterization. This inhibition could be due to alterations in angiogenic stimuli within injured corneas and/or to a decreased ability of irradiated animals to respond to such stimuli. To determine whether total body irradiation specifically affects angiogenic stimuli within injured corneal tissue, cauterized corneas from mice exposed to 900 rads of total body irradiation and from non-irradiated controls were grafted onto the chorioallantoic membranes (CAM) of chick embryos and their abilities to stimulate the ingrowth of healthy embryonic blood vessels were compared. Cauterized corneas incorporated into CAM mesenchymal tissue were invaded by blood vessels in 34.6% of the irradiated group and in 75% of the non-irradiated controls. This difference in the two groups was statistically significant (P<0.03). Total body irradiation significantly decreased the frequency of vascular invasion of cauterized corneal tissues by healthy CAM blood vessels. This finding suggests that total body irradiation can specifically affect the stimulus for angiogenesis within cauterized corneas.

Original languageEnglish (US)
Pages (from-to)1533-1542
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume26
Issue number11
StatePublished - 1985

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Effect of irradiation on vascularization of corneas grafted onto chorioallantoic membranes'. Together they form a unique fingerprint.

Cite this