Effect of intercellular communication on the selection of intraspecific human hybrids in HAT and ouabain

Cheryl M. Corsaro, Barbara R. Migeon

Research output: Contribution to journalArticlepeer-review

Abstract

Optimal conditions have been determined for selection of hybrids in HAT and ouabain (HOT) from the PEG-mediated fusion of OUAR/ HGPRT-/G6PDA and OUAs/HGPRT+/G6PDBhuman diploid fibroblasts. In addition to the OUARHGPRT+G6PDABtetraploid hybrids, several other types of HOT-resistant colonies were obtained from these fusions and identified by G6PD isozyme analysis and karyotyping as (1) hybrids contaminated with diploid parental cells, (2) symbiotic mixtures of the two types of parental cells, or (3) spontaneous OUARmutants. The mechanism for parental cell growth in HOT is most likely the transfer of HAT resistance and/or ouabain resistance through gap junctions formed between resistant and sensitive cells.

Original languageEnglish (US)
Pages (from-to)541-551
Number of pages11
JournalSomatic Cell Genetics
Volume4
Issue number5
DOIs
StatePublished - Sep 1 1978

ASJC Scopus subject areas

  • Genetics

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