Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

T. Miyazaki, K. Sueoka, A. M. Dharmarajan, S. J. Atlas, G. B. Bulkley, E. E. Wallach

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The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalJournal of Reproduction and Fertility
Issue number1
StatePublished - Jan 1 1991



  • free radicals
  • ovulation
  • progesterone
  • superoxide dismutase

ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynecology
  • Developmental Biology

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