Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

T. Miyazaki; K. Sueoka; A. M. Dharmarajan; S. J. Atlas; G. B. Bulkley; E. E. Wallach

doi:10.1530/jrf.0.0910207

Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

T. Miyazaki, K. Sueoka, A. M. Dharmarajan, S. J. Atlas, G. B. Bulkley, E. E. Wallach

School of Medicine

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.

Original language	English (US)
Pages (from-to)	207-212
Number of pages	6
Journal	Journal of Reproduction and Fertility
Volume	91
Issue number	1
DOIs	https://doi.org/10.1530/jrf.0.0910207
State	Published - 1991

Keywords

free radicals
ovulation
progesterone
superoxide dismutase

ASJC Scopus subject areas

Physiology
Embryology
Molecular Biology
Obstetrics and Gynecology
Developmental Biology

Access to Document

10.1530/jrf.0.0910207

Cite this

@article{c1c020b21a4046eea0058b31f1cabe74,

title = "Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary",

abstract = "The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.",

keywords = "free radicals, ovulation, progesterone, superoxide dismutase",

author = "T. Miyazaki and K. Sueoka and Dharmarajan, {A. M.} and Atlas, {S. J.} and Bulkley, {G. B.} and Wallach, {E. E.}",

year = "1991",

doi = "10.1530/jrf.0.0910207",

language = "English (US)",

volume = "91",

pages = "207--212",

journal = "Journal of Reproduction and Fertility",

issn = "0022-4251",

publisher = "Society for Reproduction and Fertility",

number = "1",

}

TY - JOUR

T1 - Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

AU - Miyazaki, T.

AU - Sueoka, K.

AU - Dharmarajan, A. M.

AU - Atlas, S. J.

AU - Bulkley, G. B.

AU - Wallach, E. E.

PY - 1991

Y1 - 1991

N2 - The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.

AB - The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.

KW - free radicals

KW - ovulation

KW - progesterone

KW - superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=0026018534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026018534&partnerID=8YFLogxK

U2 - 10.1530/jrf.0.0910207

DO - 10.1530/jrf.0.0910207

M3 - Article

C2 - 1995849

AN - SCOPUS:0026018534

SN - 0022-4251

VL - 91

SP - 207

EP - 212

JO - Journal of Reproduction and Fertility

JF - Journal of Reproduction and Fertility

IS - 1

ER -

Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this