Effect of influenza B virus on nutrient transport in cultured epithelial cells

M. Gurevitz, I. T. Schulze, E. M. Swierkosz, M. Q. Arens, Kathleen Schwarz

Research output: Contribution to journalArticle

Abstract

The possibility that influenza virus could induce changes in membrane permeability to nutrients ordinarily concentrated within the cell was examined. Madin-Darby canine kidney cells were infected with egg-grown influenza B virus at 37°C and pH 7.4 (a condition in which influenza virus enters cells by endocytosis). Control cells were mock-infected with allantoic fluid from chick embryos. Transport of phosphate, 2-deoxyglucose, and α-aminoisobutyric acid was measured at various intervals, 0 to 10 hours after infection. Uptake of α-aminoisobutyric acid and phosphate by infected cells was inhibited at 2 hours as compared with controls, whereas at 6 to 10 hours, the uptake of all nutrients was higher in infected cells. Infected cells preloaded with phosphate or 2-deoxyglucose did not demonstrate increased release of these nutrients. Thus, the virally induced inhibition of uptake early in infection is not a consequence of loss of membrane integrity. Transport studies were also performed in cells with prebound virus exposed to pH 5.0 for 60 seconds at 37°C and then incubated at pH 7.4, at 37°C. Under these conditions, influenza A viruses are known to enter the cell membrane by fusing directly with it and to initiate cell to cell fusion as well. We demonstrated that influenza B virus also caused cell fusion under these conditions. In contradistinction to studies described above at pH 7.4, fused, infected cells demonstrated both marked release and diminished uptake of nutrients as compared with controls. We conclude that influenza B virus does have an effect on host cell membrane permeability; the type of effect seen is markedly influenced by factors known to determine mode of virus entry into the cell.

Original languageEnglish (US)
Pages (from-to)657-664
Number of pages8
JournalLaboratory Investigation
Volume57
Issue number6
StatePublished - 1987
Externally publishedYes

Fingerprint

Influenza B virus
Cultured Cells
Epithelial Cells
Food
Cell Fusion
Phosphates
Deoxyglucose
Orthomyxoviridae
Cell Membrane Permeability
Aminoisobutyric Acids
Virus Internalization
Madin Darby Canine Kidney Cells
Membranes
Influenza A virus
Chick Embryo
Endocytosis
Infection
Ovum
Permeability
Cell Membrane

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Gurevitz, M., Schulze, I. T., Swierkosz, E. M., Arens, M. Q., & Schwarz, K. (1987). Effect of influenza B virus on nutrient transport in cultured epithelial cells. Laboratory Investigation, 57(6), 657-664.

Effect of influenza B virus on nutrient transport in cultured epithelial cells. / Gurevitz, M.; Schulze, I. T.; Swierkosz, E. M.; Arens, M. Q.; Schwarz, Kathleen.

In: Laboratory Investigation, Vol. 57, No. 6, 1987, p. 657-664.

Research output: Contribution to journalArticle

Gurevitz, M, Schulze, IT, Swierkosz, EM, Arens, MQ & Schwarz, K 1987, 'Effect of influenza B virus on nutrient transport in cultured epithelial cells', Laboratory Investigation, vol. 57, no. 6, pp. 657-664.
Gurevitz, M. ; Schulze, I. T. ; Swierkosz, E. M. ; Arens, M. Q. ; Schwarz, Kathleen. / Effect of influenza B virus on nutrient transport in cultured epithelial cells. In: Laboratory Investigation. 1987 ; Vol. 57, No. 6. pp. 657-664.
@article{d5f265e73fc24e8092f5b1663bc913aa,
title = "Effect of influenza B virus on nutrient transport in cultured epithelial cells",
abstract = "The possibility that influenza virus could induce changes in membrane permeability to nutrients ordinarily concentrated within the cell was examined. Madin-Darby canine kidney cells were infected with egg-grown influenza B virus at 37°C and pH 7.4 (a condition in which influenza virus enters cells by endocytosis). Control cells were mock-infected with allantoic fluid from chick embryos. Transport of phosphate, 2-deoxyglucose, and α-aminoisobutyric acid was measured at various intervals, 0 to 10 hours after infection. Uptake of α-aminoisobutyric acid and phosphate by infected cells was inhibited at 2 hours as compared with controls, whereas at 6 to 10 hours, the uptake of all nutrients was higher in infected cells. Infected cells preloaded with phosphate or 2-deoxyglucose did not demonstrate increased release of these nutrients. Thus, the virally induced inhibition of uptake early in infection is not a consequence of loss of membrane integrity. Transport studies were also performed in cells with prebound virus exposed to pH 5.0 for 60 seconds at 37°C and then incubated at pH 7.4, at 37°C. Under these conditions, influenza A viruses are known to enter the cell membrane by fusing directly with it and to initiate cell to cell fusion as well. We demonstrated that influenza B virus also caused cell fusion under these conditions. In contradistinction to studies described above at pH 7.4, fused, infected cells demonstrated both marked release and diminished uptake of nutrients as compared with controls. We conclude that influenza B virus does have an effect on host cell membrane permeability; the type of effect seen is markedly influenced by factors known to determine mode of virus entry into the cell.",
author = "M. Gurevitz and Schulze, {I. T.} and Swierkosz, {E. M.} and Arens, {M. Q.} and Kathleen Schwarz",
year = "1987",
language = "English (US)",
volume = "57",
pages = "657--664",
journal = "Laboratory Investigation",
issn = "0023-6837",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Effect of influenza B virus on nutrient transport in cultured epithelial cells

AU - Gurevitz, M.

AU - Schulze, I. T.

AU - Swierkosz, E. M.

AU - Arens, M. Q.

AU - Schwarz, Kathleen

PY - 1987

Y1 - 1987

N2 - The possibility that influenza virus could induce changes in membrane permeability to nutrients ordinarily concentrated within the cell was examined. Madin-Darby canine kidney cells were infected with egg-grown influenza B virus at 37°C and pH 7.4 (a condition in which influenza virus enters cells by endocytosis). Control cells were mock-infected with allantoic fluid from chick embryos. Transport of phosphate, 2-deoxyglucose, and α-aminoisobutyric acid was measured at various intervals, 0 to 10 hours after infection. Uptake of α-aminoisobutyric acid and phosphate by infected cells was inhibited at 2 hours as compared with controls, whereas at 6 to 10 hours, the uptake of all nutrients was higher in infected cells. Infected cells preloaded with phosphate or 2-deoxyglucose did not demonstrate increased release of these nutrients. Thus, the virally induced inhibition of uptake early in infection is not a consequence of loss of membrane integrity. Transport studies were also performed in cells with prebound virus exposed to pH 5.0 for 60 seconds at 37°C and then incubated at pH 7.4, at 37°C. Under these conditions, influenza A viruses are known to enter the cell membrane by fusing directly with it and to initiate cell to cell fusion as well. We demonstrated that influenza B virus also caused cell fusion under these conditions. In contradistinction to studies described above at pH 7.4, fused, infected cells demonstrated both marked release and diminished uptake of nutrients as compared with controls. We conclude that influenza B virus does have an effect on host cell membrane permeability; the type of effect seen is markedly influenced by factors known to determine mode of virus entry into the cell.

AB - The possibility that influenza virus could induce changes in membrane permeability to nutrients ordinarily concentrated within the cell was examined. Madin-Darby canine kidney cells were infected with egg-grown influenza B virus at 37°C and pH 7.4 (a condition in which influenza virus enters cells by endocytosis). Control cells were mock-infected with allantoic fluid from chick embryos. Transport of phosphate, 2-deoxyglucose, and α-aminoisobutyric acid was measured at various intervals, 0 to 10 hours after infection. Uptake of α-aminoisobutyric acid and phosphate by infected cells was inhibited at 2 hours as compared with controls, whereas at 6 to 10 hours, the uptake of all nutrients was higher in infected cells. Infected cells preloaded with phosphate or 2-deoxyglucose did not demonstrate increased release of these nutrients. Thus, the virally induced inhibition of uptake early in infection is not a consequence of loss of membrane integrity. Transport studies were also performed in cells with prebound virus exposed to pH 5.0 for 60 seconds at 37°C and then incubated at pH 7.4, at 37°C. Under these conditions, influenza A viruses are known to enter the cell membrane by fusing directly with it and to initiate cell to cell fusion as well. We demonstrated that influenza B virus also caused cell fusion under these conditions. In contradistinction to studies described above at pH 7.4, fused, infected cells demonstrated both marked release and diminished uptake of nutrients as compared with controls. We conclude that influenza B virus does have an effect on host cell membrane permeability; the type of effect seen is markedly influenced by factors known to determine mode of virus entry into the cell.

UR - http://www.scopus.com/inward/record.url?scp=0023609439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023609439&partnerID=8YFLogxK

M3 - Article

C2 - 3695411

AN - SCOPUS:0023609439

VL - 57

SP - 657

EP - 664

JO - Laboratory Investigation

JF - Laboratory Investigation

SN - 0023-6837

IS - 6

ER -